| 中文名称 | 粉防己碱 | 中文别名 | 汉防己甲素 |
| 英文名 | Tetrandrine | 英文别名 | Fanchinine; Hanfangchin A; Sinomenine A |
| 分子式 | | C38H42N2O6 | 分子量 | 622.75 | CAS登录号 | 518-34-3 | |
| 提取来源 | |
| 外观 | | 无色针状结晶(乙醚) |  | | NMR; MS | | 4℃冷藏、密封、避光 | | 2年 | | 粉防己碱 | |
| 鉴别方法 |
| 贮存条件 |
| 有效期 |
| 注意事项 |
| 溶解性 | 几乎不溶于水,石油醚,溶于乙醚和某些有机溶剂。 |
| 测定方法 | HPLC法，以十八烷基硅烷键合硅胶为填料，以甲醇-0.3%二乙胺(80:20)为流动相，检测波长为242nm。 |
| 药典测定方法 | HPLC法，以十八烷基硅烷键合硅胶为填料，以乙氰-甲醇-水-冰醋酸（40：30：30：1）为流动相，检测波长为280nm。 |
| 用途 | 用于含量测定/鉴定/药理实验等 |
文献引用： 文章标题：etrandrine mediates renal function and redox homeostasis in a streptozotocin-induced diabetic nephropathy rat model through Nrf2/HO-1 reactivation
影响因子：3.297
期刊：Annals of Translational Medicine
作者列表：Luyu Su, Ping Cao, Haiyan Wang
发表时间：2020-8-1
DOI:10.21037/atm-20-5548
主要研究成果：Abstract
Background
Diabetic nephropathy (DN) is the leading cause of morbidity and mortality in diabetic patients. Tetrandrine (Tet), a bisbenzylisoquinoline alkaloid isolated from the roots of Stephania tetrandra, possesses anti-oxidative, anti-hypertensive, anti-inflammatory capacities. In this study, the maintenance role of Tet in DN was evaluated in streptozotocin (STZ)-induced diabetic rats.
Methods
In vitro study, rats were divided into five groups (n=10): the control group, the DN model group, the Tet-treatment group (5, 15, 30 mg/kg). DN damage was assessed by levels of blood glucose, serum creatinine (CRE), proteinuria, and urea nitrogen. ELISA assay was used to detecte tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6) and IL-10 levels. Kits were used to detecte contents of malondialdehyde (MDA), lactate dehydrogenase (LDH) and superoxide dismutase (SOD). Dichlorofluorescein (DCF) staining was used to detecte reactive oxygen species (ROS). HE staining assessed pathological damage. TUNEL staining assessed tissue apoptosis. Western Blot (WB) was used to detecte levels of Ki67, Survivin, Bax, Bcl-2, caspase-3, -9, c-Myc, nuclear factor erythroid-derived 2-related factor 2 (Nrf2), p-Nrf2, and heme oxygenase-1 (HO-1).
