产品编号 | bs-0295P-PE |
英文名称 | Rabbit IgG / PE |
中文名称 | PE标记兔IgG(同型对照) |
别????名 | Rabbit Immunoglobulin G/PE Isotype Control??荧光素PE标记兔IgG(同型对照) |
Specific References??(8)?????|?????bs-0295P-PE has been referenced in 8 publications. | |
性????状 | Liquid |
浓????度 | 1.0 mg/ml |
保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
产品图片 | Blank control: SH-SY5Y. Isotype Control: Rabbit IgG-PE(bs-0295P-PE) Dilution: 1ug/Test; Primary Antibody (green line): Rabbit Anti-ChAT antibody (bs-0042R-PE) Dilution: 1ug/Test; Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Isotype control antibody (orange line) was rabbit IgG .Acquisition of 20,000 events was performed. |