上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 100ul | ￥ 480.00 | 10 |
| 1ml | ￥ 3600.00 | 10 |
| 产品编号 | bs-0368G-AF594 |
| 英文名称 | Goat Anti-Mouse IgM / AF594 antibody |
| 中文名称 | Alexa Fluor 594标记的羊抗小鼠IgM |
| 别 名 | Goat Anti-Mouse IgM (Alexa Fluor® 594); Immunoglobulin M; |
| Specific References (1) | bs-0368G-AF594 has been referenced in 1 publications. [IF=7.376] Li-Ming Yu. et al. Activation of PKG-CREB-KLF15 by melatonin attenuates Angiotensin II-induced vulnerability to atrial fibrillation via enhancing branched-chain amino acids catabolism. Free Radical Bio Med. 2021 Dec;: IF ; Mouse. | |
| 抗体来源 | Goat |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse |
| 产品应用 | IF=1:100-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Liquid |
| 浓 度 | 1.0 mg/ml |
| 免 疫 原 | Native Mouse IgM |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 | Immunoglobulin M (IgM) normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. |
文献参考： 文章标题：Lysine attenuates acute lung injury by restoring α-tubulin acetylation and ciliary activity
作者列表：Yang Wenyu, Meng Xiaoxiao, Zhu Yong, Ma Xinrun, Cao Zhuoran, Wang Mengmeng, Dai Wentao, Tao Yiming, Jian Xiangdong, Tian Rui, Yang Zhengfeng, Wang Ruilan
影响因子：7
期刊：Cell Death Discovery
发表时间：2026-3-16
DOI:10.1038/s41420-026-03025-x
文献主题：Abstract
Acute respiratory distress syndrome and pulmonary fibrosis stemming from severe acute lung injury (ALI) continue to incur high mortality due to ineffective pulmonary regeneration. While metabolic reprogramming is known to support alveolar epithelial repair, the specific role of amino acid metabolism remains enigmatic. Through integration of scRNA-seq mining analysis of human ALI samples and targeted plasma metabolomics, we identified that lysine was largely declined in injured pulmonary epithelium, accompanied by a deficiency of mitochondrial metabolism. Lysine supplementation dramatically improved survival (from 0% to 62.5% in mice), attenuated extracellular matrix deposition and alveolitis, and suppressed inflammation in murine and non-human primate ALI models. Mechanistically, lysine replenished acetyl-CoA to restore α-tubulin acetylation for rescuing ciliary TRPC1 localization, which prevented pathological STIM1-TRPC1 complex formation, thereby blocking calcium influx-reduced E-Cadherin/ZO-1 abundance in pulmonary epithelial cells. Notably, ciliogenesis preferentially occurred in SFTPC+ alveolar epithelial type II (AT2) cells; thus, lysine supplementation would promote regenerative activation of AT2 cells. Our work established lysine as a metabolic-structural orchestrator that coordinates acetyl-CoA availability to calcium homeostasis and epithelial repair through tubulin-mediated ciliary signaling.
