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环氧合酶2/前列腺素内过氧化物合成酶2抗体
产品名称:
环氧合酶2/前列腺素内过氧化物合成酶2抗体
英文名称:
Rabbit Anti-Cyclooxygenase 2 antibody
产品类别:
抗体
产品编号:
bs-0732R
保存条件:
Shipped at 4℃. Store at -20 °C for one year. Avoid
[价格]
规格 价格 库存
50ul ¥ 1180.00 10
100ul ¥ 1980.00 10
200ul ¥ 2800.00 10

产品详情

产品编号bs-0732R
英文名称Rabbit?Anti-Cyclooxygenase 2?antibody
中文名称环氧合酶2/前列腺素内过氧化物合成酶2抗体
别????名PTGS2; COX 2; COX-2; Cyclooxygenase; Cyclooxygenase2; Cyclooxygenase-2; COX2; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PGHS2; PHS 2; PHS II; PHS2 ; Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; TIS10II; PGH2_HUMAN.??
Specific References??(44)?????|?????bs-0732R has been referenced in 44 publications.
[IF=18.027]?Ke Li. et al. Oxygen Self-Generating Nanoreactor Mediated Ferroptosis Activation and Immunotherapy in Triple-Negative Breast Cancer. ACS NANO. 2023;17(5):4667–4687??WB?;??Mouse.??
[IF=6.6]?Ana Paula Girol. et al. Annexin A1 Mimetic Peptide and Piperlongumine: Anti-Inflammatory Profiles in Endotoxin-Induced Uveitis. Cells-Basel. 2021 Nov;10(11):3170??IHC?;??Rat.??
[IF=6.576]?Rong Li. et al. Integrated Analysis Reveals the Targets and Mechanisms in Immunosuppressive Effect of Mesalazine on Ulcerative Colitis. FRONT NUTR. 2022; 9: 867692??IHC,IF?;??Mouse.??
[IF=5.988]?Jing-Bei Zhang. et al. The combination of Lonicerae Japonicae Flos and Forsythiae Fructus herb-pair alleviated inflammation in liver fibrosis.. FRONT PHARMACOL. 2022 Aug;13:984611-984611??WB?;??Rat.??
[IF=5.201]?Zhong Yuyi. et al. MIR143 Inhibits Steroidogenesis and Induces Apoptosis Repressed by H3K27me3 in Granulosa Cells. Front Cell Dev Biol. 2020 Oct;8:1159??WB?;??Porcine.??
[IF=5.151]?Zhang L et al. The Protective Activities of Dietary Sea Cucumber Cerebrosides against Atherosclerosis through Regulating Inflammation and Cholesterol Metabolism in Male Mice.Mol Nutr Food Res. 2018 Aug;62(16):e1800315.??WB?;??Mouse.??
[IF=4.932]?Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFκB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096??WB?;??Human.??
[IF=4.82]?Fattori, Victor, et al. "Vinpocetine reduces diclofenac-induced acute kidney injury through inhibition of oxidative stress, apoptosis, cytokine production, and NF-κB activation in mice." Pharmacological Research (2017).??IHC-P?;??Mouse.??
[IF=4.096]?Xiaojun Pan. et al. Fibroblast growth factor-2?alleviates the capillary leakage and inflammation in sepsis. Mol Med. 2020 Dec;26(1):1-13??WB?;??Mouse.??
[IF=3.998]?Ines Fasolino. et al. 2D exfoliated black phosphorus influences healthy and cancer prostate cell behaviors. Sci Rep-Uk. 2021 Mar;11(1):1-13??IF?;??Human.??
[IF=3.998]?Naiqiang Zhu. et al. Network pharmacology integrated with experimental validation revealed the anti-inflammatory effects of Andrographis paniculata. Sci Rep-Uk. 2021 May;11(1):1-16??WB?;??Mouse.??
[IF=3.73]?Gao, Jin-Hang, et al. "Celecoxib Ameliorates Portal Hypertension of the Cirrhotic Rats through the Dual Inhibitory Effects on the Intrahepatic Fibrosis and Angiogenesis." PLOS ONE 8.7 (2013): e69309.??WB?;??Rat.??
[IF=3.69]?Yuhua Li. et al. Jiaolong capsule protects SD rats against 2,4,6-trinitrobenzene sulfonic acid induced colitis. J Ethnopharmacol. 2021 Apr;269:113716??WB?;??Rat.??
[IF=3.69]?Xiaoyan Xia. et al. Ameliorative effect of white tea from 50-year-old tree of Camellia sinensis L. (Theaceae) on kidney damage in diabetic mice via SIRT1/AMPK pathway. J Ethnopharmacol. 2021 May;272:113919??WB?;??Mouse.??
[IF=3.641]?Wenbo Ge. et al. 17β-estradiol protects sheep oviduct epithelial cells against lipopolysaccharide-induced inflammation in vitro. Mol Immunol. 2020 Nov;127:21??WB?;??Sheep.??
[IF=3.457]?Zhang J et al. Hydroxysafflor yellow A suppresses angiogenesis of hepatocellular carcinoma through inhibition of p38 MAPK phosphorylation. (2019)Biomedicine & Pharmacotherapy, 109, 806–814.??IHC?;??Mouse.??
[IF=3.405]?Tianyu Yang. et al. Material properties of degradable alloy Fe–30Mn–0.6N and its effect on ferroptosis in synoviocytes. J BIOMED MATER RES B. 2022 Sep;:??WB?;??Rabbit.??
[IF=3.37]?Zhang et al. Protective role of hydrogen-rich water on aspirin-induced gastric mucosal damage in rats. (2014) World.J.Gastroentero. 20:1614-22??IHC-P?;??Rat.??
[IF=3.368]?Liu Tang. et al. Topical Application with Conjugated Linoleic Acid Ameliorates 2, 4‐dinitrofluorobenzene‐Induced Atopic Dermatitis‐like Lesions in BALB/c Mice. Exp Dermatol. 2021 Feb;30(2):237-248??WB?;??Mouse.??
[IF=3.25]?Li, Wei, et al. "Cyclin d1 expression and the inhibitory effect of celecoxib on ovarian tumor growth in vivo." International journal of molecular sciences 11.10 (2010): 3999-4013.??WB?;??Human.??
[IF=3.241]?Zhang L et al. Eicosapentaenoic acid in the form of phospholipid exerts superior anti-atherosclerosis effects than its triglyceride form in ApoE-/-mice. Food & Function. 2019??WB?;??Mouse.??
[IF=3.118]?Possebon?L et al. Mimetic peptide AC2-26 of annexin A1 as a potential therapeutic agent to treat COPD. Int Immunopharmacol. 2018 Oct;63:270-281.??IHC-P?;??Rat.??
[IF=2.96]?Xinna Wang. et al. Hejie Zhitong prescription promotes sleep and inhibits nociceptive transmission-associated neurotransmitter activity in a rodent migraine model. Chin Med-Uk. 2020 Dec;15(1):1-12??IHC?;??Mouse.??
[IF=2.86]?Li, Wei, et al. "Combined effects of cyclooxygenase-1 and cyclooxygenase-2 selective inhibitors on ovarian carcinoma in vivo." International journal of molecular sciences 12.1 (2011): 668-681.??WB?;??Mouse.??
[IF=2.81]?Liu et al. Sinomenine sensitizes multidrug-resistant colon cancer cells (Caco-2) to doxorubicin by downregulation of MDR-1 expression. (2014) PLoS.On. 9:e98560??WB?;??Human.??
[IF=2.72]?Weifeng Li. et al. Schisandrin improves lipopolysaccharide-induced acute lung injury by inhibiting the inflammatory response in vivo and in?vitro. 2022 Mar 21??WB?;??Mouse,Human.??
[IF=2.65]?Shengqi Niu. et al. Jatrorrhizine Alleviates DSS-Induced Ulcerative Colitis by Regulating the Intestinal Barrier Function and Inhibiting TLR4/MyD88/NF-κB Signaling Pathway.. EVID-BASED COMPL ALT. 2022 Sep;2022:3498310-3498310??WB?;??Mouse.??
[IF=2.535]?Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523.??WB?;??Human.??
[IF=2.42]?Li, Weifeng, et al. "Protective effect of sanguinarine on LPS-induced endotoxic shock in mice and its effect on LPS-induced COX-2 expression and COX-2 associated PGE< sub> 2 release from peritoneal macrophages." International Immunopharmacology (2014).??IHC-P?;??Mouse.??
[IF=2.408]?Jingru Qin. et al. In-silico analysis identifies the anti-liver injury targets of diammonium glycyrrhizinate: validated in perfluorooctanoic acid-lesioned mouse model. 2022 Mar 31??IHC?;??Mouse.??
研究领域肿瘤??心血管??免疫学??信号转导??合成与降解??新陈代谢??
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human,Mouse,Rat (predicted: Dog,Pig,Cow,Rabbit,Sheep,Recombinant fragment,)
产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量65kDa
细胞定位细胞浆?
性????状Liquid
浓????度1mg/ml
免?疫?原KLH conjugated synthetic peptide derived from human Cyclooxygenase 2:?501-604/604?
亚????型IgG
纯化方法affinity purified by Protein A
缓?冲?液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
产品介绍Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

Function:
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.

Subunit:
Homodimer.

Subcellular Location:
Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Post-translational modifications:
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.

Similarity:
Belongs to the prostaglandin G/H synthase family.Contains 1 EGF-like domain.

SWISS:
P35354

Gene ID:
5743

Database links:

Entrez Gene: 5743?Human

Entrez Gene: 19225?Mouse

Entrez Gene: 29527?Rat

Omim: 600262?Human

SwissProt: P35354?Human

SwissProt: Q05769?Mouse

SwissProt: P35355?Rat

Unigene: 196384?Human

Unigene: 292547?Mouse

Unigene: 44369?Rat



合成与降解(Synthesis and Degradation)
COX-2 前列腺素氧化环化酶-2是前列腺素合成的关键酶之一,为诱导性酶,他参与细胞对生长因子、肿瘤促进因子和细胞因子的反应,这些因子均能诱导COX2表达,它也参与前列腺素的合成。
COX2在80-90%的人结肠腺癌中表达显著增高,而COX1表达却不变。
产品图片
Sample:
Lane 1: Recombinant human COX2 protein
Primary: Anti-Cyclooxygenase 2 (bs-0732R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 40 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclooxygenase 2) Polyclonal Antibody, Unconjugated (bs-0732R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclooxygenase 2) Polyclonal Antibody, Unconjugated (bs-0732R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃for 20 min; Incubation: Anti-Cyclooxygenase 2 Polyclonal Antibody, Unconjugated(bs-0732R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): HepG2 (blue).
Primary Antibody (green line): Rabbit Anti-Cyclooxygenase 2/FITC Conjugated antibody (bs-0732R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-Cyclooxygenase 2 antibody (bs-0732R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-Cyclooxygenase 2 antibody (bs-0732R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-Cyclooxygenase 2 antibody (bs-0732R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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