产品分类/PRODUCT CLASSIFICATION
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | ￥ 1180.00 | 10 |
| 100ul | ￥ 1980.00 | 10 |
| 200ul | ￥ 2800.00 | 10 |
| 产品编号 | bs-10177R |
| 英文名称 | Rabbit?Anti-Calbindin?antibody |
| 中文名称 | 钙结合蛋白D28K抗体 |
| 别????名 | CAB27; CALB 1; CALB; CALB1; Calbindin 1 28kDa; Calbindin-D-28K; Calbindin D28; D 28K; D28K; Vitamin D dependent calcium binding protein; Vitamin D dependent calcium binding protein avian type; Vitamin D dependent calcium binding protein avian-type; Vitamin D-dependent calcium-binding protein; CALB1_HUMAN. ?? |
| 研究领域 | 细胞生物??免疫学??神经生物学??生长因子和激素??结合蛋白?? |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Pig,Cow,Horse,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 29kDa |
| 细胞定位 | 细胞核?细胞浆?细胞外基质? |
| 性????状 | Liquid |
| 浓????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human Calbindin:?41-150/261? |
| 亚????型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓?冲?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq, Jul 2008] Function: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Subunit: Interacts with RANBP9. Similarity: Belongs to the calbindin family. Contains 5 EF-hand domains. SWISS: P05937 Gene ID: 793 Database links: Entrez Gene: 793 Human Entrez Gene: 12307 Mouse SwissProt: P05937 Human SwissProt: P12658 Mouse |
| 产品图片 |  Sample: kidney (mouse) Lysate at 40 ug brain (Mouse) Lysate at 40 ug Primary: Anti-Calbindin(bs-10177R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 29 kD Observed band size: 29 kD  Sample: Lane 1: Mouse Brain Lysates Lane 2: Mouse Cerebellum Lysates Lane 3: Mouse Kidney Lysates Lane 4: Rat Kidney Lysates Primary: Anti-Calbindin (bs-10177R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29kDa Observed band size: 29kDa  Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin ) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin ) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
