细胞角蛋白7抗体-上海雅吉生物科技有限公司

规格	价格	库存
50ul	￥ 1180.00	10
100ul	￥ 1980.00	10
200ul	￥ 2800.00	10

产品编号	bs-1744R
英文名称	Rabbit?Anti-CK7?antibody
中文名称	细胞角蛋白7抗体
别????名	CK 7; CK-7; Cytokeratin 7; Cytokeratin-7; Cytokeratin7; D15Wsu77e; K2C7; K2C7_HUMAN; K7; Keratin 55k type ii cytoskeletal; Keratin 7; Keratin simple epithelial type 1 k7; Keratin type II cytoskeletal 7; Keratin type ii cytoskletal 7; Keratin, 55K type II cytoskeletal; Keratin, simple epithelial; Keratin, simple epithelial type I, K7; Keratin, type II cytoskeletal 7; Keratin-7; Keratin7; KRT 7; Krt2-7; KRT7; MGC11625; MGC129731; mgc3625; Sarcolectin; SCL; Type ii mesothelial keratin k7; Type-II keratin Kb7??
	Specific References??(1)?????\|?????bs-1744R has been referenced in 1 publications. [IF=2.24]?Zhang, Ni-Ni, et al. "Functional regeneration of irradiated salivary glands with human amniotic epithelial cells transplantation." International Journal of Clinical and Experimental Pathology? 6.10 (2013): 2039-2047.??Mouse.?? PubMed:24133581
研究领域	肿瘤??细胞生物??信号转导??
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse,Rat
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, Flow-Cyt=1ug/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	54kDa
细胞定位	细胞浆?
性????状	Liquid
浓????度	1mg/ml
免?疫?原	KLH conjugated synthetic peptide derived from the middle of mouse CK7:?251-350/469?
亚????型	IgG
纯化方法	affinity purified by Protein A
缓?冲?液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] Function: Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Subunit: Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. Post-translational modifications: Arg-20 is dimethylated, probably to asymmetric dimethylarginine. Similarity: Belongs to the intermediate filament family. SWISS: Q9DCV7 Gene ID: 110310 Database links: Entrez Gene: 3855?Human Entrez Gene: 110310?Mouse Entrez Gene: 300242?Rat Omim: 148059?Human SwissProt: P08729?Human SwissProt: Q9DCV7?Mouse SwissProt: Q6IG12?Rat Unigene: 411501?Human Unigene: 670221?Human Unigene: 289377?Mouse Unigene: 7913?Rat 结构蛋白（Structural Proteins） CK-7是一种 54KDa 的中间丝蛋白，存在于大多数正常组织的腺上皮和移行上皮细胞中。该抗体与多种良/恶性上皮性肿瘤反应。腺癌中的卵巢、乳腺、肺的腺癌呈阳性反应，而胃肠道的腺癌阴性。移行细胞肿瘤、前列腺癌也呈阳性反应。通常认为 CK7是腺癌和移行上皮细胞癌的比较特异性的标志。
产品图片	Sample: Placenta (Mouse) Lysate at 30 ug Lung (Mouse) Lysate at 30 ug Primary: Anti-Cytokeratin 7 (bs-1744R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 54 kD Observed band size: 54 kD Sample: Large intestine (Mouse) Lysate at 30 ug Primary: Anti- Cytokeratin 7 (bs-1744R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 54 kD Observed band size: 54 kD Sample: Lane 1: Mouse Urinary bladder tissue lysates Lane 2: Mouse Breast tissue lysates Lane 3: Mouse Placenta tissue lysates Lane 4: Mouse trachea tissue lysates Lane 5: Rat Urinary bladder tissue lysates Lane 6: Rat Placenta tissue lysates Lane 7: Human Hela cell lysates Lane 8: Human HepG2 cell lysates Lane 9: Human Siha cell lysates Lane 10: Human Huvec cell lysates Primary: Anti-CK7 (bs-1744R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 52 kDa Sample: Large intestine (Mouse) Lysate at 40 ug Large intestine(Rat) Lysate at 40 ug Primary: Anti- Cytokeratin 7 (bs-1744R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD Sample: Embryo (Mouse) Lysate at 40 ug Primary: Anti- Cytokeratin 7 (bs-1744R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cytokeratin 7 Polyclonal Antibody, Unconjugated(bs-1744R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Polyclonal Antibody, Unconjugated (bs-1744R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK7) Polyclonal Antibody, Unconjugated (bs-1744R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cytokeratin 7) polyclonal Antibody, Unconjugated (bs-1744R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control:Hela. Primary Antibody (green line): Rabbit Anti-Cytokeratin 7 antibody (bs-1744R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

联系我们