热休克蛋白70抗体-上海雅吉生物科技有限公司

规格	价格	库存
50ul	￥ 1180.00	10
100ul	￥ 1980.00	10
200ul	￥ 2800.00	10

产品编号	bs-0244R
英文名称	Rabbit?Anti-HSP70?antibody
中文名称	热休克蛋白70抗体
别????名	HSP70; HSP-70; HSP 70; Heat shock 70 kDa protein 1; heat shock 70kDa protein 1A; Heat shock 70kDa protein 1B; Heat shock induced protein; heat shock protein 70; HSP70 1; HSP70 2; HSP70.1; HSP72; HSPA1; HSPA1A; HSPA1B; XXbac BCX40G17.3 001; Heat shock 70 kDa protein 1A/1B; HSP71A_HUMAN; Hspa1a; Hsp70-1; HSP72; Hspa1; Hspa1b; Heat shock 70 kDa protein 1A/1B; shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2.??
	Specific References??(17)?????\|?????bs-0244R has been referenced in 17 publications. [IF=10.652]?Yanan Hu. et al. Preparation of photothermal responsive and ROS generative gold nanocages for cancer therapy. Chem Eng J. 2021 Oct;421:129744??WB?;??Human.?? PubMed:10.1016/j.cej.2021.129744 [IF=10.435]?Wan, Guoyun. et al. Gene augmented nuclear-targeting sonodynamic therapy via Nrf2 pathway-based redox balance adjustment boosts peptide-based anti-PD-L1 therapy on colorectal cancer. J Nanobiotechnol. 2021 Dec;19(1):1-26??IF?;??Mouse.?? PubMed:34715867 [IF=5.818]?Hehe Du. et al. Cromileptes altivelis microRNA Transcriptome Analysis upon Nervous Necrosis Virus (NNV) Infection and the Effect of cal-miR-155 on Cells Apoptosis and Virus Replication. VIRUSES-BASEL. 2022 Oct;14(10):2184??FC?;??Cromileptes altivelis.?? PubMed:36298739 [IF=5.738]?Lee Jisu. et al. Alpha-2-macroglobulin as a novel diagnostic biomarker for human bladder cancer in urinary extracellular vesicles. FRONT ONCOL. 2022 Sep;0:4727??WB?;??Human.?? PubMed:36176383 [IF=5.696]?Jiang-Tao Fan. et al. Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway. Neoplasia. 2021 Jul;23:692??WB?;??Human.?? PubMed:34153644 [IF=5.108]?Wang Y et al. Excessive Cu2+ deteriorates arsenite-induced apoptosis in chicken brain and resulting in immunosuppression, not in homeostasis. Chemosphere.?2019 Sep 4;239:124758.??WB?;??Chicken.?? PubMed:31514009 [IF=4.411]?Hong-Mei Li. et al. Anti-Cancer Properties of Ginkgolic Acids in Human Nasopharyngeal Carcinoma CNE-2Z Cells via Inhibition of Heat Shock Protein 90. Molecules. 2021 Jan;26(21):6575??WB?;??Human.?? PubMed:34770993 [IF=3.74]?Zheng, Jian, et al. "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." Journal of Neurosurgery (2016): 1-9.??WB?;??Rat.?? PubMed:27739937 [IF=3.738]?Wang, Rui. et al. Protective Effect of Manganese on Apoptosis and Mitochondrial Function of Heat-Stressed Primary Chick Embryonic Myocardial Cells. 2021 Nov 15??WB?;??Chicken.?? PubMed:34779997 [IF=3.118]?Liu?J et al. Impacts of simultaneous exposure to arsenic (III) and copper (II) on inflammatory response, immune homeostasis, and heat shock response in chicken thymus. Int Immunopharmacol. 2018 Nov;64:60-68. ??WB?;??Chicken.?? PubMed:30145471 [IF=3.038]?Oyagbemi AA et al. Quercetin attenuates hypertension induced by sodium fluoride via reduction in oxidative stress and modulation of HSP 70/ERK/PPARγ signaling pathways. (2018) Sep 01??IHC?;??Rat.?? PubMed:30171731 [IF=2.984]?Xia Wang. et al. Distinct expression and localization patterns of HSP70 in developmental reproductive organs of rams. Gene. 2020 Nov;760:145029??WB,IF?;??Sheep.?? PubMed:32758578 [IF=2.323]?Xinglin Liu. et al. Effects of Dietary Zearalenone Exposure on the Growth Performance, Small Intestine Disaccharidase, and Antioxidant Activities of Weaned Gilts. Animals-Basel. 2020 Nov;10(11):2157??WB,IHC?;??Pig.?? PubMed:33228146 [IF=2.245]?Dahan Yang. et al. Tandem Mass Tag-Based Quantitative Proteomic Analysis of Chicken Bursa of Fabricius Infected With Reticuloendotheliosis Virus. Front Vet Sci. 2021; 8: 666512??WB?;??Chicken.?? PubMed:34113672 [IF=1.561]?Li L et al. Avian leukosis virus subgroup J and reticuloendotheliosis virus coinfection induced TRIM62 regulation of the actin cytoskeleton. J Vet Sci. 2020 May;21(3):e49.??WB?;??Chicken.?? PubMed:32476322 [IF=1.561]?Ling Li. et al. Avian leukosis virus subgroup J and reticuloendotheliosis virus coinfection induced TRIM62 regulation of the actin cytoskeleton. J Vet Sci. 2020 May; 21(3): e49??WB?;??Chicken.?? PubMed:32476322 [IF=0]?Zhou M et al. Comparative study of stress response, growth and development of uteri in post‐weaning gilts challenged with zearalenone and estradiol benzoate. J Anim Physiol Anim Nutr (Berl). 2019 Sep 4.??WB&IHC?;??Pig.?? PubMed:31483545
研究领域	肿瘤??细胞生物??免疫学??信号转导??
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,Mouse,Rat (predicted: Chicken,Cow,Rabbit,Sheep)
产品应用	WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1ug/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理论分子量	70kDa
细胞定位	细胞浆?
性????状	Liquid
浓????度	1mg/ml
免?疫?原	KLH conjugated synthetic peptide derived from human HSP70:?500-600/641?
亚????型	IgG
纯化方法	affinity purified by Protein A
缓?冲?液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
产品介绍	This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]. Function: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Subunit: Component of the CatSper complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with TSC2. Interacts with IRAK1BP1. Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed. Interacts with DNAJC7. Interacts with CHCHD3. Subcellular Location: Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Tissue Specificity: HSPA1B is testis-specific. Similarity: Belongs to the heat shock protein 70 family. SWISS: P0DMV8 Gene ID: 3303 Database links: Entrez Gene: 281825?Cow Entrez Gene: 3303?Human Entrez Gene: 3304?Human Entrez Gene: 15511?Mouse Entrez Gene: 193740?Mouse Entrez Gene: 24472?Rat Entrez Gene: 294254?Rat Omim: 140550?Human Omim: 603012?Human SwissProt: Q27975?Cow SwissProt: P0DMV8?Human SwissProt: P0DMV9?Human SwissProt: P17879?Mouse SwissProt: Q61696?Mouse SwissProt: Q07439?Rat Unigene: 274402?Human Unigene: 719966?Human Unigene: 728810?Human Unigene: 1950?Rat Unigene: 228225?Rat HSP-70是细胞受应激原刺激后诱导产生的一组应激蛋白，与肿瘤发生、增殖及分化有关。环境和病生理性应激原均可导致肌体合成一组应急蛋白既热休克蛋白。许多热休克蛋白，包括HSP70家族成员，均参与蛋白的变性-复性、折叠-解折叠、运输-易位、活化-非活化和分泌等过程。HSP70与类固醇受体、肌动蛋白、P53等蛋白密切相关。HSP70还参与热应激原、细胞毒药物和其他损伤所引起的应急反应，对机体起一定的保护作用。
产品图片	Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Epididymis (Mouse) Lysate at 40 ug Lane 3: Seminal vesicle (Mouse) Lysate at 40 ug Lane 4: Liver (Mouse) Lysate at 40 ug Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 6: Testis (Rat) Lysate at 40 ug Lane 7: Spleen (Rat) Lysate at 40 ug Lane 8: Hela (Human) Cell Lysate at 30 ug Lane 9: Jurkat (Human) Cell Lysate at 30 ug Lane 10: HepG2 (Human) Cell Lysate at 30 ug Lane 11: A549 (Human) Cell Lysate at 30 ug Lane 12: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-HSP70 (bs-0244R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 68 kD Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (bs-0244R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (bs-0244R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP70) Polyclonal Antibody, Unconjugated (bs-0244R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP70) polyclonal Antibody, Unconjugated (bs-0244R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control:A549. Primary Antibody (green line): Rabbit Anti-HSP70 antibody (bs-0244R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:A549. Primary Antibody (green line): Rabbit Anti-HSP70 antibody (bs-0244R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:293T. Primary Antibody (green line): Rabbit Anti-HSP70 antibody (bs-0244R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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