上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 50ul | ￥ 1180.00 | 10 |
| 100ul | ￥ 1980.00 | 10 |
| 200ul | ￥ 2800.00 | 10 |
| 产品编号 | bs-4200R |
| 英文名称 | Rabbit?Anti-Selenium Binding Protein 1?antibody |
| 中文名称 | 硒结合蛋白1抗体 |
| 别????名 | 56 kDa selenium binding protein; 56 kDa selenium-binding protein; hSBP; LPSB; SBP; SBP1; SBP1_HUMAN; SBP56; Selenbp1; Selenbp2; Selenium binding protein 2; Selenium binding protein1; Selenium-binding protein 1; SELNBP1; SP56.?? |
 | Specific References??(1)?????|?????bs-4200R has been referenced in 1 publications. [IF=6.107]?Xiao-Hu Zhao. et al. Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients. ISCIENCE. 2023 May;:106831??WB?;??Human.?? |
| 研究领域 | 肿瘤??免疫学??神经生物学??信号转导?? |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Dog,Cow,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test, ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 52kDa |
| 细胞定位 | 细胞核?细胞浆?细胞膜? |
| 性????状 | Liquid |
| 浓????度 | 1mg/ml |
| 免?疫?原 | KLH conjugated synthetic peptide derived from human SBP1/Selenium Binding Protein 1:?401-472/472? |
| 亚????型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓?冲?液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | Selenium is an essential trace element that confers tolerance to toxicity arising through exposure to heavy metals or other reactive xenobiotics. Selenium exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. Both effects are attributed to selenium-binding proteins. Selenium binding protein 1 is down-regulated in lung adenocarcinoma, colorectal cander and ovarian cancer. It is two-fold upregulated in the brains of patients suffering from schizophrenia, and is therefore a biomarker for this disease. Function: Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport. Subunit: Interacts with USP33. Subcellular Location: Nucleus. Cytoplasm, cytosol. Membrane; Peripheral membrane protein. Note=May associate with Golgi membrane. May associate with the membrane of autophagosomes. Tissue Specificity: Present in liver and colon (at protein level). Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the selenium-binding protein family. SWISS: Q13228 Gene ID: 8991 Database links: Entrez Gene: 8991 Human Entrez Gene: 20341 Mouse Omim: 604188 Human SwissProt: Q13228 Human SwissProt: P17563 Mouse Unigene: 632460 Human Unigene: 16617 Rat |
| 产品图片 |  Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti- Selenium Binding Protein 1 (bs-4200R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD  Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-Selenium Binding Protein 1 (bs-4200R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD  Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti- Selenium Binding Protein 1 (bs-4200R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD  Sample: Liver (Mouse) Lysate at 40 ug Kidney (Mouse) Lysate at 40 ug Primary: Anti- Selenium Binding Protein 1 (bs-4200R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD  Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti- Selenium Binding Protein 1 (bs-4200R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD  Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Selenium Binding Protein 1) Polyclonal Antibody, Unconjugated (bs-4200R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDNF) Polyclonal Antibody, Unconjugated (bs-1024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Selenium Binding Protein 1) Polyclonal Antibody, Unconjugated (bs-4200R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Selenium Binding Protein 1) Polyclonal Antibody, Unconjugated (bs-4200R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-Selenium Binding Protein 1 antibody (bs-4200R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488R Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.  Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-Selenium Binding Protein 1 antibody (bs-4200R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
