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APC anti-human CD4 Antibody
产品名称：
APC anti-human CD4 Antibody
产品类别：
抗体
产品编号：
317415
产品应用：
317415
[价格]
规格 价格 库存
25tests ￥ 33 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding.?Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
  3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
  5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
  6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
  7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
  8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
  9. Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations
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  13. Dushyanthen S, et al. 2017. Nat Commun. 10.1038/s41467-017-00728-9. PubMed
  14. Wang Z, et al. 2021. Cell Mol Immunol. 18:2188. PubMed
  15. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  16. Acerbi E, et al. 2016. Sci Rep. 6:23128. PubMed
  17. Altman K 2006. J Immunol . 177:1721. PubMed
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  20. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
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  23. Arif S, et al. 2020. Diabetologia. 63(6):1186-1198.. PubMed
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  25. Jiang H, et al. 2021. Oncoimmunology. 10:1943180. PubMed
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  27. Zhang J, et al. 2022. Nature. 609:369. PubMed
  28. Johnson A, et al. 2016. mSphere. 1: e00288-16. PubMed
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  31. Jafari M, et al. 2014. J Virol. 88:5062. PubMed
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  33. Frencher J, et al. 2014. J Leukoc Biol. 96:957. PubMed
  34. Li N, et al. 2020. Oncoimmunology. 9:1824643. PubMed
  35. Li N, et al. 2021. Cell Rep Med. 2:100297. PubMed
  36. Dahal S, et al. 2022. Retrovirology. 19:18. PubMed
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  44. Su Y, et al. 2020. Cell. 1479:183. PubMed
  45. Mujib S, et al. 2017. JCI Insight. 2:e93687. PubMed
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RRID
AB_571944 (BioLegend Cat. No. 317415) AB_571945 (BioLegend Cat. No. 317416)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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