Alexa Fluor® 488 anti-human CD4 Antibody
- 产品名称：
- Alexa Fluor® 488 anti-human CD4 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 317419
- 产品应用：
- 317419
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human peripheral T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding.?Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317453 and 317454).
In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
- Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
- Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
- Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
- Linder J, et al. 1987. Am. J. Pathol. 127:1.
- Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
- Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
- Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
- Product Citations
-
- Demirkhanyan L, et al. 2012. J Biol Chem. 287:28821. PubMed
- Johnson RF, et al. 2021. Viruses. 13:. PubMed
- Rademacher MJ, et al. 2021. Sci Rep. 11:8321. PubMed
- Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
- Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
- Abe S, et al. 2020. JCI Insight. 5:00. PubMed
- Kraig E, et al. 2018. Exp Gerontol. 105:53. PubMed
- Zhou H, et al. 2022. EMBO Rep. 23:e53475. PubMed
- Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed
- Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
- Billing F, et al. 2021. ACS Appl Mater Interfaces. 13:55534. PubMed
- Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
- Maehara T, et al. 2018. Life Sci Alliance. 1:e201800050. PubMed
- Singh A, et al. 2020. Cell Rep. 32:108153. PubMed
- Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
- Sharei A, et al. 2015. PLoS One. 10:118803. PubMed
- RRID
- AB_571938 (BioLegend Cat. No. 317419) AB_571939 (BioLegend Cat. No. 317420)
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 55 kD
- Distribution
-
T cell subset, majority of thymocytes, monocytes/macrophages
- Function
- MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
- Ligand/Receptor
- MHC class II molecules, HIV gp120, IL-16
- Cell Type
- Macrophages, Monocytes, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
- Gene ID
- 920 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org
