PerCP/Cyanine5.5 anti-human CD3 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-human CD3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 317335
- 产品应用：
- 317335
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.
Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Li B, et al. 2005. Immunology 116:487.
- Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
- Alter G, et al. 2008. J. Virol. 82:9668. PubMed
- Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
- Pinto JP, et al. 2010. Immunology. 130:217. PubMed
- Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
- Product Citations
-
- Aschmoneit N, et al. 2021. J Immunother Cancer. 9:. PubMed
- Lee E, et al. 2021. Sci Rep. 11:14841. PubMed
- Sim J, et al. 2019. MAbs. 11:1036. PubMed
- Kasper M, et al. 2021. Elife. 10:. PubMed
- Wang J, et al. 2020. Cell. 183:1264. PubMed
- Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
- Narsale A, Moya R, Robertson H 2016. Data Brief. 8: 1348-51. PubMed
- Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
- Billing F, et al. 2021. ACS Appl Mater Interfaces. 13:55534. PubMed
- Zhang Z, et al. 2021. Acta Pharm Sin B. 11:1965. PubMed
- Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
- Bulgarelli J, et al. 2021. Front Immunol. 12:778459. PubMed
- Aschmoneit N, et al. 2022. Oncoimmunology. 11:2028961. PubMed
- Wu X, et al. 2021. Front Immunol. 12:731767. PubMed
- Aschmoneit N, et al. 2021. Sci Rep. 11:13880. PubMed
- Schoofs T et al. 2019. Immunity. 50(6):1513-1529 . PubMed
- Mudd PA, et al. 2022. Cell. 185:603. PubMed
- Alonso R, et al. 2022. Blood Adv. :. PubMed
- Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
- Collinson–Pautz MR, et al. 2019. Leukemia. 33:2195. PubMed
- Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed
- Albayati Z, et al. 2017. Scand J Immunol. 86:396. PubMed
- Ott M, et al. 2020. JCI Insight. 5(17):134386. PubMed
- RRID
- AB_2561627 (BioLegend Cat. No. 317335) AB_2561628 (BioLegend Cat. No. 317336)
- Structure
- Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
- Distribution
-
Mature T and NK T cells, thymocyte differentiation
- Function
- Antigen recognition, signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen bound to MHC
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
- Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
- Lanier L, et al. 1986. J. Immunol. 137:2501.
- Gene ID
- 916 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
