PE anti-human CD3 Antibody
- 产品名称：
- PE anti-human CD3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 317307
- 产品应用：
- 317307
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.
Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Li B, et al. 2005. Immunology 116:487.
- Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
- Alter G, et al. 2008. J. Virol. 82:9668. PubMed
- Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
- Pinto JP, et al. 2010. Immunology. 130:217. PubMed
- Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
- Product Citations
-
- Dam T, et al. 2021. Front Physiol. 11:613367. PubMed
- Lugo-Reyes SO, et al. 2021. J Clin Immunol. 41:1291. PubMed
- Huanosta-Murillo E, et al. 2021. Front Immunol. 12:668369. PubMed
- O'Neill A, et al. 2014. Clin Cancer Res. 20:678. PubMed
- Peng S et al. 2019. Cell Rep. 28(10):2728-2738 . PubMed
- Rau A, et al. 2021. MAbs. 1902034:13. PubMed
- Giesecke C, et al. 2017. Cytometry A. 10.1002/cyto.a.23250. PubMed
- Man F, et al. 2019. Mol Ther. 27:219. PubMed
- Wang LT, et al. 2020. Cell Rep. 32:108188. PubMed
- Trinklein ND, et al. 2019. MAbs. 11:639. PubMed
- Parajuli P, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
- Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
- Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
- Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
- Kelly DL, et al. 2018. Psychiatry Res. 269:517. PubMed
- Imle A, et al. 2019. Nat Commun. 10:2144. PubMed
- RRID
- AB_571912 (BioLegend Cat. No. 317307) AB_571913 (BioLegend Cat. No. 317308)
- Structure
- Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
- Distribution
-
Mature T and NK T cells, thymocyte differentiation
- Function
- Antigen recognition, signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen bound to MHC
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
- Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
- Lanier L, et al. 1986. J. Immunol. 137:2501.
- Gene ID
- 916 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
