1 ?g/mL of antibody antibody in PBS, for plate coating protocols; 10 ?g/mL of OKT3 antibody for soluble antibody activation methods

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.⁴ The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

This antibody is intended for activation and expansion of CD3 T cells in research and ex vivo cell culture applications. The antibody was GMP manufactured under serum-free conditions, including serum-free hybridoma cell culture, without additional animal or human-derived materials.

OKT3 antibody can be used in combination with anti-human CD28 antibodies to efficiently activate T cells when used at 1 ?g/mL of antibody in PBS, for plate coating protocols. Plates should be coated for 2 hours in a tissue culture incubator, or overnight at 4°C. For soluble antibody activation protocols we recommend 10 ?g/mL of OKT3 antibody and 5 ?g/mL of anti-human CD28.

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3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
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GMP Ultra-LEAF? antibodies. BioLegend GMP Ultra-LEAF? antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research or ex vivo cell processing use. Not for use in diagnostic or therapeutic procedures.?Our processes include:

• Batch-to-batch consistency? ??
• Material traceability
• Documented procedures
• Documented employee training
• Equipment maintenance and monitoring records
• Lot-specific certificates of analysis
• Quality audits per ISO 13485:2016
• QA review of released products

BioLegend GMP Ultra-LEAF? antibodies are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12

Mature T and NK T cells, thymocyte differentiation

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.