Biotin anti-human CD3 Antibody
- 产品名称：
- Biotin anti-human CD3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 317319
- 产品应用：
- 317319
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.
Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
- Li B, et al. 2005. Immunology 116:487.
- Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
- Alter G, et al. 2008. J. Virol. 82:9668. PubMed
- Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
- Pinto JP, et al. 2010. Immunology. 130:217. PubMed
- Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
- Product Citations
-
- Zhang DKY, et al. 2020. Nature Protocols. 15(3):773-798.. PubMed
- Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
- Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
- Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
- Dong R, et al. 2020. EMBO J. 39:e104730. PubMed
- Xu X, et al. 2021. Elife. 10:. PubMed
- Motsch V, et al. 2019. Sci Rep. 2.658333333. PubMed
- Serwas NK, et al. 2019. Nat Commun. 2.573611111. PubMed
- Sun J et al. 2018. Cell stem cell. 23(3):355-369 . PubMed
- Allegrezza M, et al. 2016. Cancer Res . 76: 2561 - 2572. PubMed
- Zheng Y, et al. 2021. Nat Commun. 12:3580. PubMed
- Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
- RRID
- AB_10918432 (BioLegend Cat. No. 317319) AB_10916519 (BioLegend Cat. No. 317320)
- Structure
- Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
- Distribution
-
Mature T and NK T cells, thymocyte differentiation
- Function
- Antigen recognition, signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen bound to MHC
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
- Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
- Lanier L, et al. 1986. J. Immunol. 137:2501.
- Gene ID
- 916 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
