APC anti-mouse TCR Vβ5.1, 5.2 Antibody
- 产品名称：
- APC anti-mouse TCR Vβ5.1, 5.2 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 139505
- 产品应用：
- 139505
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Murine T cell hybridoma 2HB51.8
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Induction of proliferation of V?5.1+?and V?5.2+?T cells2, 3?and?in vivo?depletion of V?5+?T cells4.
- Application References
(PubMed link indicates BioLegend citation) -
- Kanagawa O,?et al.?1991.?J. Immunol.?147:1307. (FC)
- Kanagawa O,?et al.?1992.?J. Immunol.?149:9. (Activ)
- Woodland DL,?et al.?1993.?J. Exp. Med.?177:433. (Activ)
- Gelber C,?et al.?1992.?Cancer Res.?52:6507. (Deplete)
- Product Citations
-
- Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
- Kashyap AS, et al. 2019. Cell Rep. 28:3367. PubMed
- Liu X, et al. 2020. Nature. . PubMed
- Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
- RRID
- AB_10897800 (BioLegend Cat. No. 139505) AB_10933250 (BioLegend Cat. No. 139506)
- Structure
- Member of the immunoglobulin superfamily, Vβ5.1and 5.2 are variants of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
- Distribution
-
Expressed on a subset of TCR αβ+ T cells.
- Function
- Recognition of peptides presented by the MHC molecules, responsible for T cell mediated immune responses.
- Ligand/Receptor
- MHC-Peptide complex
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Marrack P,?et al.?2008.?Annu. Rev. Immunol.?26:171.?
2. Sim GK and Augustin AA. 1985.?Cell?42:89.?
3. Mami-Chouaib F,?et al.?2002.?Immunol. Rev.?188:114.
- Gene ID
- 21577 View all products for this Gene ID
- UniProt
- View information about TCR Vb5.1, 5.2 on UniProt.org
