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Purified anti-human IgM Antibody
产品名称:
Purified anti-human IgM Antibody
产品类别:
抗体
产品编号:
314502
产品应用:
314502
[价格]
规格 价格 库存
100µg ¥ 101 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per 106 in 100 ?l staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations
  1. Mclaurin JD, et al. 2019. Mol Biol Cell. 30:1610. PubMed
  2. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  3. Pyfrom SC, et al. 2020. J Immunol. 205:595. PubMed
  4. Jung Y, et al. 2021. Nat Commun. 12:3872. PubMed
  5. Kolhatkar N, et al. 2015. J Exp Med. 212: 1663-1677. PubMed
  6. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  7. Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed
  8. Georg P, et al. 2022. Cell. 185:493. PubMed
  9. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  10. Waugh KA, et al. 2020. Cell Reports. 29(7):1893-1908.e4.. PubMed
  11. Martin E, et al. 2020. JCI Insight. :5. PubMed
RRID
AB_493003 (BioLegend Cat. No. 314502)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
3507 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

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