PerCP/Cyanine5.5 anti-human IgM Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-human IgM Antibody
- 产品类别：
- 抗体
- 产品编号：
- 314511
- 产品应用：
- 314511
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per 106 cells in 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.
Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
- Product Citations
-
- Carre C, et al. 2021. iScience. 24:102970. PubMed
- Lagou V et al. 2018. Cell reports. 25(3):798-810 . PubMed
- Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed
- Català-Moll F, et al. 2021. Nucleic Acids Res. 49:5057. PubMed
- Yang Z, et al. 2018. Front Immunol. 9:2613. PubMed
- van der Burg M, et al. 2019. Front Immunol. 10:246. PubMed
- Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
- Kohn L, et al. 2014. J Immunol. 192:5050. PubMed
- Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
- Aggarwal C, et al. 2021. J Virol. 95:e0061021. PubMed
- Flint S, et al. 2016. Haematologica. 101: 698 - 706. PubMed
- van der Velden V, et al. 2017. J Immunol Methods. 10.1016/j.jim.2017.03.011. PubMed
- Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
- Rother M, et al. 2016. J Immunol. 197: 441 - 448. PubMed
- Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed
- van Dongen JJM, et al. 2019. Front Immunol. 10:1271. PubMed
- RRID
- AB_961367 (BioLegend Cat. No. 314511) AB_2076098 (BioLegend Cat. No. 314512)
