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Pacific Blue™ anti-human IgM Antibody
产品名称:
Pacific Blue™ anti-human IgM Antibody
产品类别:
抗体
产品编号:
314513
产品应用:
314513
[价格]
规格 价格 库存
25µg ¥ 165 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations
  1. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  2. Ghraichy M, et al. 2021. Elife. 10:. PubMed
  3. Hubbard N, et al. 2016. Blood. 127: 2513 - 2522. PubMed
  4. Catera R, et al. 2017. Mol Med. 23:1. PubMed
  5. Levy Y, et al. 2021. iScience. 24:102711. PubMed
  6. Rouers A, et al. 2021. iScience. 24:102482. PubMed
  7. Wiedemann A, et al. 2020. Nat Commun. 3.048611111. PubMed
RRID
AB_10574306 (BioLegend Cat. No. 314513) AB_10574307 (BioLegend Cat. No. 314514)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
3507 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

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