FITC anti-human IgM Antibody
- 产品名称：
- FITC anti-human IgM Antibody
- 产品类别：
- 抗体
- 产品编号：
- 314506
- 产品应用：
- 314506
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 188 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.
Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
- Product Citations
-
- Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
- Watanabe A, et al. 2019. Cell. 177:1124. PubMed
- Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
- Muir L, et al. 2018. Wellcome Open Res. 2:97. PubMed
- Fenwick C, et al. 2022. Nat Microbiol. 7:1376. PubMed
- Lindner JM, et al. 2019. Immunity. 50:668. PubMed
- Zost SJ, et al. 2020. Nat Med. 26:1422. PubMed
- Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
- Zost SJ, et al. 2020. bioRxiv. . PubMed
- Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
- Ros F, et al. 2020. MAbs. 12:1846900. PubMed
- Jürchott K, et al. 2016. PLoS One. 11: 0150812. PubMed
- Lisboa F, et al. 2011. J Biol Chem. 286:7577. PubMed
- Benhammou JN, et al. 2020. Dig Dis Sci. 66:2394. PubMed
- Nicolò A, et al. 2022. Front Immunol. 13:1016263. PubMed
- Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
- Cizmeci D, et al. 2021. eLife. 10:00. PubMed
- Fenwick C, et al. 2021. Cell Rep. 37:109814. PubMed
- Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
- Ju L,et al. 2017. Sci Rep.. 10.1038/s41598-017-13793-3. PubMed
- Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
- Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
- Song S, et al. 2021. Front Immunol. 12:705140. PubMed
- RRID
- AB_493009 (BioLegend Cat. No. 314506)
