Biotin anti-human IgE Antibody
- 产品名称:
- Biotin anti-human IgE Antibody
- 产品类别:
- 抗体
- 产品编号:
- 325503
- 产品应用:
- 325503
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
ELISA, IHC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
- Application References
(PubMed link indicates BioLegend citation) -
- Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed.
- Product Citations
-
- Farkas AM, et al. 2019. J Allergy Clin Immunol. 143:335. PubMed
- Li T, et al. 2018. J Biol Chem. 293:1243. PubMed
- RRID
- AB_830848 (BioLegend Cat. No. 325503) AB_830849 (BioLegend Cat. No. 325504)