Purified anti-MMP2 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 229	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human MMP-2, amino acids (Ala30-Cys660) (Accession# NP_004521.1) with a C-terminal His tag and a linker sequence expressed in 293E cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration of 2 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of MMP-2 recombinant protein ranging from 10 to 0.156 ng/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture:?The purified M6303D01 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M6310C11 antibody (Cat. No. 532001) as the detecting antibody.

Note: For testing human MMP-2 in serum or plasma, BioLegend's LEGEND MAX? Kits (Cat. No. 444607 & 444608) are specially developed and recommended.

Product Citations

Brezovakova V, et al. 2022. Cells. 11:. PubMed

RRID

AB_2566322 (BioLegend Cat. No. 679902)

Antigen Details

Structure: Monomer. Human MMP2 contains 660 amino acids with a predicted molecular weight of 73.9 kD. Upon activation by p-aminophenylmercuric acetate (APMA), it has a predicted molecular weight of 62 kD.
Distribution: MMP2 is expressed by fibroblasts, endothelial cells, alveolar epithelial cells, and macrophages.
Function: MMP2 is a metalloproteinase that specifically cleaves type IV collagen, which is the major structural component of basement membranes. MMP2 is thought to contribute to the invasion and the metastasis of various human malignancies.
Interaction: Fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans, decorin, osteopontin, and IBP-3.
Ligand/Receptor: TIMPs, CLL7, and thrombospondin 1.
Cell Type: Endothelial cells, Epithelial cells, Fibroblasts, Macrophages
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Enzymes and Regulators
Antigen References: 1. Nagase H and Woessner JF. 1999. J. Biol. Chem. 274:21491.
2. Martignetti JA, et al. 2001. Nat. Genet. 28:261.
3. Itoh T, et al. 1997. J. Biol. Chem. 272:22389.
4. Passlick B, et al. 2000. Clin. Cancer Res. 6:3944.
5. Qian Q, et al. 2010. Cancer Invest. 28:661.
6. Hoikkala S, et al. 2006. Cancer Lett. 236:125.
7. Yamamura T, et al. 2002. Cancer 35:249.
Gene ID: 4313 View all products for this Gene ID
UniProt: View information about MMP-2 on UniProt.org

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