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Biotin anti-mouse IL-25 (IL-17E) Antibody
产品名称:
Biotin anti-mouse IL-25 (IL-17E) Antibody
产品类别:
抗体
产品编号:
538104
产品应用:
538104
[价格]
规格 价格 库存
100µg ¥ 265 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Mouse IL-25
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 1 - 8 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-25 (IL-17E) recombinant protein ranging from 1600 to 25 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The biotinylated M3704D11 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the pure M3706F07 antibody (Cat. No. 676304) as the capture antibody and Recombinant Recombinant Mouse IL-25 (IL-17E) (ELISA Std.) (Cat. No. 579809).

RRID
AB_2814449 (BioLegend Cat. No. 538104)

Antigen Details

Structure
Heterodimer
Distribution

IL-25 is produced by mast cells, epithelial cells, alveolar macrophages, microglia, and Th2 cells

Function
IL-25 directly promotes Th2 cell differentiation and the expression of IL-4, IL-5, IL-13, CCL11 (eotaxin), and therefore, lung eosinophilia. IL-25 enhances IL-9 production by differentiated Th9 cells
Interaction
CD4+ NKT cells, macrophages, endothelial cells, eosinophils, and Th2 and Th9 cells
Ligand/Receptor
IL-25 binds to IL-17RB
Antigen References
  1. Hurst SD,?et al.?2002.?J. Immunol.?169:443.
  2. Ikeda K,?et al.?2003.?Blood?101:3594.
  3. Kang CM,?et al.?2005.?Am. J. Respir. Cell. Mol. Biol.?33:290.
  4. Kleinschek ME,?et al.?2007.?J. Exp. Med.?204:161.
  5. Terashima A,?et al.?2008.?J. Exp. Med.?205:2727.
  6. Angkasekwinai P,?et al.?2010.?Nat. Immunol.?11:250
Gene ID
140806 View all products for this Gene ID
UniProt
View information about IL-25 on UniProt.org

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