Direct-Blot™ HRP anti-Granzyme B Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µL	￥ 340	1

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Granzyme B produced in the 293E cell line.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB -?Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1,000-1:10.000. The optimal dilution should be determined by titration for each individual assay of interest.

25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Application Notes: This antibody can be used for at least 20 western blots.
RRID: AB_2687057 (BioLegend Cat. No. 674603)

Structure: 247 amino acids with a molecular weight of approximately 28 kD
Distribution: Cytotoxic T cells, NK cells, and neutrophils.
Function: Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8⁺ T lymphocytes with ConA / IL-2 and CD4⁺ T lymphocytes with anti-CD3/CD28 or anti-CD3/CD46.
Interaction: Targets of CTL and NK cells.
Cell Type: B cells, Dendritic cells, Neutrophils, NK cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: 1. Edwards KM,?et al.?1999.?J. Biol. Chem.?274:30468.
2. Grossman WJ,?et al.?2004.?Blood?104:2840.
3. Heusel JW,?et al.?1994.?Cell?76:977.
4. Schmid J and Weissmann C. 1987.?J. Immunol.?139:250.
5. Trapani JA,?et al.?1988.?Proc. Natl. Acad. Sci. USA?85:6924.
6. Hiebert PR and Granville DJ. 2012.?Trends Mol. Med.?18:732.
7. Saito Y,?et al.?2011.?J. Cardiol.?57:141.
8. Ewen CL,?et al.?2012.?Cell Death Differ.?1:28.
Gene ID: 3002 View all products for this Gene ID
UniProt: View information about Granzyme B on UniProt.org