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Purified anti-MMP9 Antibody
产品名称:
Purified anti-MMP9 Antibody
产品类别:
抗体
产品编号:
681802
产品应用:
681802
[价格]
规格 价格 库存
100µg ¥ 229 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human MMP-9, amino acids (Ala20-Asp707) (Accession# NP_004985.2), is expressed with a C-terminal 8His tag and a linker sequence in the 293E cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture?- Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration of 4.0??g/mL is recommended. To obtain a linear standard curve, serial dilutions of MMP-9?recombinant protein ranging from 5?to 0.078 ng/mL is recommended for each ELISA plate.?It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is only tested on recombinant proteins. It may not be able to detect endogenous proteins from cell or tissue lysates by western blotting (WB), where the expression levels may be too low.

The purified M2101G05 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M2108F07 antibody (Cat. No. 532101) as the detecting antibody.

Note:?For testing human MMP-9 in serum or plasma, BioLegend's LEGEND MAX? Kit (Cat. No. 444907) is specially developed and recommended.

RRID
AB_2566497 (BioLegend Cat. No. 681802)

Antigen Details

Distribution

MMP9 is expressed by alveolar macrophages, granulocytes, fibroblasts, and fibrosarcoma cells.

Function
Degradation of extracellular matrix (ECM) and connective tissue proteins, angiogenesis, tissue remodeling, and cancer metastasis. Regulates chemokine activity. Inhibited by TIMPs and α2-macroglobulin (inhibitor of MMPs in bodily fluids).
Interaction
ECM proteins.
Ligand/Receptor
TIMPs.
Cell Type
Fibroblasts, Granulocytes, Macrophages
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References

1. Chakrabarti S, Patel KD. 2005. Exp. Lung Res. 31:599.
2. Klein T, Bischoff R. 2011. Amino Acids 41:271.
3. Raffetto JD, Khalil RA. 2008. Biochem. Pharmacol. 75:346.
4. Bourboulia D, Stetler-Stevenson WG. 2010. Semin. Cancer Biol. 20:161.
5. Vilen ST, et al. 2013. Scientific World Journal 920595.

Gene ID
4318 View all products for this Gene ID
UniProt
View information about MMP-9 on UniProt.org

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