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Purified anti-mouse PCSK9 Antibody
产品名称:
Purified anti-mouse PCSK9 Antibody
产品类别:
抗体
产品编号:
532402
产品应用:
532402
[价格]
规格 价格 库存
500µg ¥ 581 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
In-house recombinant mouse PCSK9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 2 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of PCSK9 recombinant protein ranging from 4000 to 62.5 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

ELISA Capture: The purified M114G10 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5251 antibody as the detecting antibody.
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Note: For testing mouse PCSK9 in serum, plasma, cell culture supernatant, cell lysate, tissue homogenate, and other biological fluids, BioLegend's LEGEND MAX? Kits (Cat. No. 443207 & 443208) are specially developed and recommended.

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RRID
AB_2686955 (BioLegend Cat. No. 532402)

Antigen Details

Structure
Monomer. The molecular mass of a full-length and mature protein is 74.8 kD and 53.7 kD, respectively.
Distribution

Hepatocytes, renal cells, neuroepithelioma, and Schwann cells.

Function
PCSK9 regulates LDLR, VLDLR, APOER, and APOER2 levels, and therefore, LDLc levels. PCSK9 is regulated by SREBP-2.
Interaction
Hepatocytes.
Ligand/Receptor
LDLR, VLDLR, APOB, LRP1, and LRP8.
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Enzymes and Regulators
Antigen References

1.?Seidah NG,?et al.?2003.?Proc. Natl. Acad. Sci. USA?100:928.
2.?Naureckiene S,?et al.?2003.?Arch. Biochem. Biophys.?420:55.
3.?Costet P,?et al.?2008.?Trends Biochem. Sci.?33:426.
4.?Grefhorst A,?et. al.?2008.?J. Lipid Res.?49:1303.
5.?Tavori H,?et al.?2013.?Circulation?127:2403.
6.?Denis M,?et al.?2012.?Circulation?125:894.
7.?Tibolla G,?et al.?2011.?Nutr. Metab. Cardiovasc. Dis.?21:835.

Gene ID
100102 View all products for this Gene ID
UniProt
View information about PCSK9 on UniProt.org

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