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Biotin anti-human NGAL (Lipocalin-2) Antibody
产品名称:
Biotin anti-human NGAL (Lipocalin-2) Antibody
产品类别:
抗体
产品编号:
503304
产品应用:
503304
[价格]
规格 价格 库存
500µg ¥ 469 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human NGAL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.15 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 2500 to 39.1 pg/mL is recommended for each ELISA plate.

It is recommended that the reagent be titrated for optimal performance.

Application Notes

ELISA Detection: The biotinylated M049D6 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M0415B7 (Cat. No. 661902) antibody as the capture antibody.

?

Note: For testing human NGAL in cell culture supernatant, serum, plasma, urine, saliva, and other biological fluids, BioLegend's LEGEND MAX? Kits (Cat. No. 443407 & 443408) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Iqbal N, et al. 2013. Expert Opin. Med Diagn. 7:209.
  2. Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663.
  3. Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832.
  4. Flo TH, et al. 2004. Nature 432:917
  5. Goetz DH, et al. 2002. Mol. Cell. 10:1033.
  6. Leung L, et al. 2012. PLoS One 7:e46677
  7. Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425.
RRID
AB_2650710 (BioLegend Cat. No. 503304)

Antigen Details

Structure
193 amino acid monomer with a predicted molecular weight of approximately 22 kD.
Distribution

Secreted by epithelial cells, macrophages, neutrophils. Increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.

Function
Apoptosis, innate immunity, renal development.
Interaction
Disulfide-linked with MMP9
Ligand/Receptor
SLC22A17 (24p3R)
Cell Type
Epithelial cells, Macrophages, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Gene ID
3934 View all products for this Gene ID
UniProt
View information about NGAL on UniProt.org

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