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Purified anti-mouse NGAL (Lipocalin-2) Antibody
产品名称:
Purified anti-mouse NGAL (Lipocalin-2) Antibody
产品类别:
抗体
产品编号:
532202
产品应用:
532202
[价格]
规格 价格 库存
500µg ¥ 581 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
In-house recombinant mouse NGAL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA -?Quality tested
IP -?Verified

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 1.5 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 1000 to 15.6 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

The purified M047A10 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M0417A8 antibody as the detecting antibody.

For testing mouse NGAL in cell culture supernatant, cell lysate, serum, plasma, urine, and other biological fluids, BioLegend's specially developed?LEGEND MAX? Kits are recommended.

RRID
AB_2650711 (BioLegend Cat. No. 532202)

Antigen Details

Structure
195 amino acid monomer with a predicted molecular weight of approximately 23 kD.
Distribution

Secreted by epithelial cells, macrophages, neutrophils; increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.

Function
Apoptosis, innate immunity, renal development.
Interaction
Disulfide-linked with MMP9.
Ligand/Receptor
Apoptosis, innate immunity, renal development.
Cell Type
Epithelial cells, Macrophages, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1.?Iqbal N,?et al.?2013.?Expert Opin.Med. Diagn.?7:209.
2.?Singer E,?et al.?2013.?Acta. Physiol. (Oxf)?207:663.
3.?Palazzuoli A,?et al.?2012.?Clin. Chim. Acta.?413:1832.
4.?Flo TH,?et al.?2004.?Nature?432:917.
5.?Goetz DH,?et al.?2002.?Mol. Cell.?10:1033.
6.?Leung L,?et al.?2012.?PLoS One?7:e46677.
7.?Kjeldsen L,?et al.?1993.?J. Biol. Chem.?268:10425.

Gene ID
16819 View all products for this Gene ID
UniProt
View information about NGAL on UniProt.org

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