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Biotin anti-mouse NGAL (Lipocalin-2) Antibody
产品名称:
Biotin anti-mouse NGAL (Lipocalin-2) Antibody
产品类别:
抗体
产品编号:
532304
产品应用:
532304
[价格]
规格 价格 库存
100µg ¥ 265 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
In-house recombinant mouse NGAL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.1 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 1000 to 15.6 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

The biotinylated M0417A8 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M047A10 (Cat. No. 532202) antibody as the capture antibody.

Note: For testing mouse NGAL in cell culture supernatant, serum, plasma, urine and other biological fluids, BioLegend's LEGEND MAX? Kits (Cat. No. 443707 & 443708) are specially developed and recommended.

RRID
AB_2686952 (BioLegend Cat. No. 532304)

Antigen Details

Structure
195 amino acid monomer with a predicted molecular weight of approximately 23 kD.
Distribution

Secreted by epithelial cells, macrophages, neutrophils; increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.

Function
Apoptosis, innate immunity, renal development.
Interaction
Disulfide-linked with MMP9.
Ligand/Receptor
SLC22A17 (24p3R)
Cell Type
Epithelial cells, Macrophages, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Iqbal N,?et al.?2013.?Expert Opin. Med Diagn.?7:209.?
2. Singer E,?et al.?2013.?Acta. Physiol. (Oxf)?207:663.?
3. Palazzuoli A,?et al.?2012.?Clin. Chim. Acta.?413:1832.?
4. Flo TH,?et al.?2004.?Nature?432:917.?
5. Goetz DH,?et al.?2002.?Mol. Cell.?10:1033.?
6. Leung L,?et al.?2012.?PLoS One?7:e46677.?
7. Kjeldsen L,?et al.?1993.?J. Biol. Chem.?268:10425.

Gene ID
16819 View all products for this Gene ID
UniProt
View information about NGAL on UniProt.org

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