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Biotin anti-human LIF Antibody
产品名称:
Biotin anti-human LIF Antibody
产品类别:
抗体
产品编号:
530301
产品应用:
530301
[价格]
规格 价格 库存
50µg ¥ 176 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human LIF, amino acids (Ser23-Phe202) (Accession# NP_002300.1), was expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 2.0 ?g/ml of the detection antibody was utilized to generate the example standard curve for human LIF. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: To measure human LIF, this antibody can be used as a detection antibody in a sandwich ELISA format and paired with the purified clone Poly5233 antibody as the capture antibody. Recombinant Human LIF (ELISA Std.) can be used as the protein standard.

Note: For testing human LIF in serum, plasma, or supernatant, BioLegend’s LEGEND MAX? Human LIF ELISA Kit is specially developed and recommended.

RRID
AB_2566783 (BioLegend Cat. No. 530301)

Antigen Details

Structure
Four-helix bundle fold.
Distribution
Expressed in the trophectoderm of the developing embryo and activated CD4+ T cells.
Function
LIF is a pleiotropic cytokine involved in cellular development, inflammation, immunology, and neoplasia.
Interaction
Embryonic stem cells.
Ligand/Receptor
LIF Rα, gp130
Cell Type
T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Metcalf D. 1991. Int. J. Cell Cloning 2:95-108.
2. Zhang JG, et al. 1998. J. Biol. Chem. 17:10798.
3. Kamohara H, et al. 2007. Int. J. Oncol. 4:977.
4. Marshall MK, et al. 1994. Endocrinology 135:141.
5. Vogiagis D, Salamonsen LA. 1999. J. Endocrinol. 160:181.
6. Zouein FA, et al. 2013. Eur. Cytokine Netw. 24:11.
7. Mathieu ME, et al. 2012. Stem Cell Rev. 8:1.
8. Hu W, et al. 2007. Nature 450:721.
9. Trouillas M, et al. 2009. Eur. Cytokine Netw. 20:51.
10. Metcalfe SM. 2011. Genes Immun. 3:157.

Gene ID
3976 View all products for this Gene ID
UniProt
View information about LIF on UniProt.org

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