HRP anti-His Tag Antibody
- 产品名称:
- HRP anti-His Tag Antibody
- 产品类别:
- 抗体
- 产品编号:
- 652503
- 产品应用:
- 652503
- Verified Reactivity
- All species (6x His tag epitope)
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated His-tag peptide
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solution with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
- Application
-
ELISA - Quality tested
WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by direct ELISA assay. For direct ELISA, a concentration range of 0.01 - 1.0 ?g per ml is recommended. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the end-user titrate each lot of reagents for optimal performance.
- Application References
(PubMed link indicates BioLegend citation) -
- Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
- Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
- Product Citations
-
- Hay CE, et al. 2020. J Pharmacol Exp Ther. 16:374. PubMed
- Bojadzic D, et al. 2021. Front Pharmacol. 11:600372. PubMed
- Bojadzic D, et al. 2021. ACS Infect Dis. 7:1519. PubMed
- Wang HC, et al. 2020. Antimicrob Agents Chemother. 65:. PubMed
- Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed
- Chen X, et al. 2021. Nat Commun. 12:5506. PubMed
- Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
- Zhao Y, et al. 2019. Cancer Biol Ther. 0.925. PubMed
- Zhang X, et al. 2021. Nat Chem. 13:626. PubMed
- Chen X, et al. 2020. bioRxiv. . PubMed
- Houben B, et al. 2020. EMBO J. e102864:39. PubMed
- Harrasser M, et al. 2022. Breast Cancer Res. 24:39. PubMed
- Hay CE, et al. 2018. PLoS One. 13:29. PubMed
- RRID
- AB_2734520 (BioLegend Cat. No. 652503) AB_2563555 (BioLegend Cat. No. 652504)