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HRP anti-His Tag Antibody
产品名称:
HRP anti-His Tag Antibody
产品类别:
抗体
产品编号:
652503
产品应用:
652503
[价格]
规格 价格 库存
25µg ¥ 112 1

产品详情

Product Details

Verified Reactivity
All species (6x His tag epitope)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solution with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
Application

ELISA - Quality tested
WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by direct ELISA assay. For direct ELISA, a concentration range of 0.01 - 1.0 ?g per ml is recommended. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application References

(PubMed link indicates BioLegend citation)
  1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Product Citations
  1. Hay CE, et al. 2020. J Pharmacol Exp Ther. 16:374. PubMed
  2. Bojadzic D, et al. 2021. Front Pharmacol. 11:600372. PubMed
  3. Bojadzic D, et al. 2021. ACS Infect Dis. 7:1519. PubMed
  4. Wang HC, et al. 2020. Antimicrob Agents Chemother. 65:. PubMed
  5. Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed
  6. Chen X, et al. 2021. Nat Commun. 12:5506. PubMed
  7. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  8. Zhao Y, et al. 2019. Cancer Biol Ther. 0.925. PubMed
  9. Zhang X, et al. 2021. Nat Chem. 13:626. PubMed
  10. Chen X, et al. 2020. bioRxiv. . PubMed
  11. Houben B, et al. 2020. EMBO J. e102864:39. PubMed
  12. Harrasser M, et al. 2022. Breast Cancer Res. 24:39. PubMed
  13. Hay CE, et al. 2018. PLoS One. 13:29. PubMed
RRID
AB_2734520 (BioLegend Cat. No. 652503) AB_2563555 (BioLegend Cat. No. 652504)

Antigen Details

Function
Recombinant poly-histidine tag detection
Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about His Tag on UniProt.org

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