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PE anti-His Tag Antibody
产品名称:
PE anti-His Tag Antibody
产品类别:
抗体
产品编号:
362603
产品应用:
362603
[价格]
规格 价格 库存
100tests ¥ 335 1

产品详情

Product Details

Verified Reactivity
Human, Mouse, Rat, All species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.

Clone J095G46 has been verified for?immunocytochemistry (ICC).

Product Citations
  1. Tasker C, et al. 2021. AAPS J. 23:26. PubMed
  2. Li Z, et al. 2019. Cell Mol Immunol. 0.897222222. PubMed
  3. Huang J, et al. 2021. Immunity. 54(4):673-686.e4. PubMed
  4. Wei C, et al. 2020. Nat Metab. 1.049305556. PubMed
  5. Le Gall CM, et al. 2021. Bioconjug Chem. 32:301. PubMed
  6. Yang J, et al. 2020. Nature. 586:572. PubMed
  7. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  8. Tong P, et al. 2021. Cell. :. PubMed
  9. Huang HC, et al. 2021. EBioMedicine. 74:103712. PubMed
  10. Scheu AHA, et al. 2021. Nat Commun. 0.997916667. PubMed
  11. Pivniouk V, et al. 2022. J Allergy Clin Immunol. 149:923. PubMed
  12. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  13. Long Y, et al. 2021. Biochem Biophys Res Commun. 571:152. PubMed
  14. van der Schoot JMS, et al. 2019. Sci Adv. 5:eaaw1822. PubMed
  15. Sokal A, et al. 2021. Immunity. S1074-7613:00396. PubMed
  16. Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed
  17. Wang R, et al. 2021. Immunity. 54:1611. PubMed
  18. Zhang M, et al. 2022. iScience. 25:104490. PubMed
RRID
AB_2563634 (BioLegend Cat. No. 362603)

Antigen Details

Structure
6 repeat His amino acid
Function
Epitope tag
Biology Area
Cell Biology, Immunology
Antigen References
  1. Zhao C, et al. 2010. Anal Biochem. 399:237
  2. Salichs E, et al. 2009. PLoS Genet. 5:e1000397
  3. Terpe K. 2003. Appl. Microbiol. Biotechnol. 60:523
  4. Lindner, P, et al. 1997. Biotechniques. 22:140
  5. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  6. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Gene ID
NA
UniProt
View information about His Tag on UniProt.org

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