Purified anti-human IL-8 Antibody
- 产品名称：
- Purified anti-human IL-8 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 511501
- 产品应用：
- 511501
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-8 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection: The purified H8A5 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated E8N1 (Cat. No. 511404) antibody as the detection antibody.
Note: For testing human IL-8 in serum or plasma, BioLegend's ELISA Max? Sets are specially developed and recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Sauer U, et al. 2011. Anal Biochem. 419:46. PubMed
- Domnanich P, et al. 2011. Biosens Bioeletron. 26:2259. PubMed
- Sauer U, et al. 2012. J Immunol Methods. 378:44. PubMed
- Product Citations
-
- Gogalic S, et al. 2018. Biosensors (Basel). 9:1593. PubMed
- Sauer U, et al. 2011. Anal Biochem. 419:46. PubMed
- Domnanich P, et al. 2011. Biochem Biophys Res Commun. 26:2559. PubMed
- Sauer U, et al. 2012. J Immunol Methods. 378:44. PubMed
- RRID
- AB_893455 (BioLegend Cat. No. 511501) AB_893456 (BioLegend Cat. No. 511502)
- Structure
- CXC chemokine; 8.4 kD (Mammalian)
- Ligand/Receptor
- CXCR1 (CDw128); CXCR2
- Bioactivity
- Neutrophil/basophil chemoattractant; activates neutrophils, angiogenic factor
- Cell Sources
- Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes
- Cell Targets
- Neutrophils, basophils, lymphocytes
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Baggiolini M, et al. 1994. Adv. Immunol. 55:97.
3. Schr?der J, et al. 1992. Immunology Ser. 57:387.
4. Zwahlen R, et al. 1993. Intl. Rev. Expt. Pathol. 34:27.
- Regulation
- Upregulated by IL-1, IL-7, IL-17, IFN-γ, TNF-α, leukoregulin; downregulated by IL-4, TGF-?, glucocorticoids, vitamin D3
- Gene ID
- 3576 View all products for this Gene ID
- UniProt
- View information about IL-8 on UniProt.org
