Purified anti-Apo E, 109-116 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 276	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

WB - Quality tested
Direct ELISA - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 ?g per ml.?For Direct ELISA applications, a concentration range of 0.004 - 0.4 ?g/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application References (PubMed link indicates BioLegend citation)

Mahley RW. 2016. Arterioscler Thromb Vasc Biol. 36: 1305-1315.
Riedel BC, et al. 2016. J Steroid Biochem Mol Biol. 160:134-47.
Tai LM, et al. 2016. Acta Neuropathol. 131(5):709-23.
Kim J, et al. 2014. Mol. Cells. 37:767
Holtzman DM, et al. 2012. Cold Spring Harb. Perspect. Med. 2:a006312

RRID

AB_2728597 (BioLegend Cat. No. 852701) AB_2728598 (BioLegend Cat. No. 852702)

Antigen Details

Structure

Human APO E is a 299 amino acid protein with a molecular mass of 32 kD, and exists as 3 major isoforms, APO E2, APO E3, and APO E4.

Distribution

Tissue sources: Ubiquitously expressed. Highest levels in liver, brain, spleen, adrenal gland, and kidney.

Cellular distribution: Plasma membrane, nucleus, endoplasmic reticulum, golgi apparatus, endosome, and extracellular.

Function

Apo E is involved in lipoprotein metabolism as well as the transport of fat-soluble vitamins, and cholesterol. Apo E also forms complexes with amyloid beta peptides leading to its uptake and degradation by microglia.

Interaction

Tau, amyloid beta

Ligand/Receptor

LDL receptor, SORL1

Biology Area

Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation

Molecular Family

Apolipoproteins

Antigen References