Purified anti-mouse Ly-6A/E (Sca-1) Antibody
- 产品名称：
- Purified anti-mouse Ly-6A/E (Sca-1) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 122501
- 产品应用：
- 122501
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- mouse pre-T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F, IHC-P, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1,?immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.
The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7
- Application References
(PubMed link indicates BioLegend citation) -
- Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
- Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
- van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
- Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
- van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
- Brandi C, et al. 2009. Exp Eye Res. 89:209. PubMed
- Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
- Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
- ?
- Product Citations
-
- Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
- Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
- Goldsmith JR, et al. 2020. Nat Commun. 11:2591. PubMed
- Lee JH, et al. 2022. iScience. 25:104166. PubMed
- Yao L, et al. 2021. J Bone Miner Res. 36:1159. PubMed
- Gronowicz G, et al. 2017. Tissue Eng Part A. 23:1490. PubMed
- Felthaus O, et al. 2010. Neurosci Lett. 471:179. PubMed
- Brandl C, et al. 2009. Exp Eye Res. 89:209. PubMed
- Martin K,et al. 2017. J Immunol.. 10.4049/jimmunol.1601522. PubMed
- Shah K, et al. 2022. Nat Commun. 13:1827. PubMed
- Umemoto T, et al. 2017. EMBO J. 36:2390. PubMed
- Hsieh HY, et al. 2020. Cancer Sci. 111:2400. PubMed
- Nascimento DC, et al. 2017. Nat Commun. 8:14919. PubMed
- Helbling PM, et al. 2019. Cell Rep. 29:3313. PubMed
- Ishii H, et al. 2018. Nat Commun. 2.188194444. PubMed
- Rogers P, Driessnack M, and Schwartz E. 2017. PLoS One. 10.1371/journal.pone.0181985. PubMed
- Zhang C, et al. 2018. Cell Res. 28:323. PubMed
- Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
- RRID
- AB_756186 (BioLegend Cat. No. 122501) AB_756187 (BioLegend Cat. No. 122502)
- Structure
- Ly-6 multigene family, 18 kD
- Distribution
-
Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
- Function
- Regulates B and T cell responses
- Cell Type
- B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
- Biology Area
- Immunology, Stem Cells
- Antigen References
-
1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.
- Gene ID
- 110454 View all products for this Gene ID
- UniProt
- View information about Ly-6A/E on UniProt.org
