PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody
- 产品名称：
- PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 122513
- 产品应用：
- 122513
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- mouse pre-T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1,?immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.
The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7
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- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
- Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
- van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
- van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
- Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
- Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
- Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
- Hara T, et al. 2012. J. Immunol. 189:1577. PubMed
- Product Citations
-
- Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
- Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed
- Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
- Guo B, et al. 2018. Nat Med. 24:360. PubMed
- Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
- Hara T, et al. 2012. J Immunol. 189:1577. PubMed
- Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
- Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
- Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
- Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
- Bues J, et al. 2022. Nat Methods. 19:323. PubMed
- Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
- Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
- Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
- Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
- Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
- Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
- Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
- Kalafati L, et al. 2020. Cell. 183:771. PubMed
- Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
- Thürmer M, et al. 2022. Nat Commun. 13:2982. PubMed
- Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
- Muzumdar S, et al. 2019. Am J Pathol. 189:568. PubMed
- Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
- Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
- Luo H, et al. 2019. Cell Rep. 26:945. PubMed
- RRID
- AB_756198 (BioLegend Cat. No. 122513) AB_756199 (BioLegend Cat. No. 122514)
- Structure
- Ly-6 multigene family, 18 kD
- Distribution
- Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
- Function
- Regulates B and T cell responses
- Cell Type
- Hematopoietic stem and progenitors, T cells, B cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Antigen References
-
1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.
- Gene ID
- 110454 View all products for this Gene ID
- UniProt
- View information about Ly-6A/E on UniProt.org
