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APC/Fire™ 750 anti-mouse Ly-6A/E (Sca-1) Ant
产品名称:
APC/Fire™ 750 anti-mouse Ly-6A/E (Sca-1) Ant
产品类别:
抗体
产品编号:
122525
产品应用:
122525
[价格]
规格 价格 库存
25µg ¥ 106 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse pre-T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire? 750 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire? 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1,?immunohistochemical staining of frozen sections2?and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.

The two Sca-1 recognizing clones?D7?and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.

Application References

(PubMed link indicates BioLegend citation)
  1. Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
  2. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
  3. van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
  4. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  5. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
  6. Brandi C, et al. 2009. Exp Eye Res. 89:209. PubMed
  7. Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
  8. Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
RRID
AB_2687352 (BioLegend Cat. No. 122525) AB_2687353 (BioLegend Cat. No. 122526)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells.

Function
Regulates B and T cell responses.
Cell Type
Hematopoietic stem and progenitors, B cells, T cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL,?et al.?1989.?Immunol. Rev.?111:195.?
2. Morrison SJ,?et al.?1994.?Immunity?1:661.?
3. Spangrude GJ,?et al.?1988.?J. Immunol.?141:3697.?
4. Malek T,?et al.?1986.?J. Exp. Med.?164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A Ly-6E on UniProt.org

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