APC/Fire™ 750 anti-mouse Ly-6A/E (Sca-1) Ant
- 产品名称:
- APC/Fire™ 750 anti-mouse Ly-6A/E (Sca-1) Ant
- 产品类别:
- 抗体
- 产品编号:
- 122525
- 产品应用:
- 122525
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse pre-T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire? 750 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC -?Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire? 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1,?immunohistochemical staining of frozen sections2?and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.
The two Sca-1 recognizing clones?D7?and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.
- Application References
(PubMed link indicates BioLegend citation) -
- Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
- Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
- van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
- Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
- van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
- Brandi C, et al. 2009. Exp Eye Res. 89:209. PubMed
- Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
- Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
-
- RRID
- AB_2687352 (BioLegend Cat. No. 122525) AB_2687353 (BioLegend Cat. No. 122526)
- Structure
- Ly-6 multigene family, 18 kD
- Distribution
-
Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells.
- Function
- Regulates B and T cell responses.
- Cell Type
- Hematopoietic stem and progenitors, B cells, T cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Antigen References
-
1. Rock KL,?et al.?1989.?Immunol. Rev.?111:195.?
2. Morrison SJ,?et al.?1994.?Immunity?1:661.?
3. Spangrude GJ,?et al.?1988.?J. Immunol.?141:3697.?
4. Malek T,?et al.?1986.?J. Exp. Med.?164:709.
- Gene ID
- 110454 View all products for this Gene ID
- UniProt
- View information about Ly-6A Ly-6E on UniProt.org