APC anti-mouse Ly-6A/E (Sca-1) Antibody
- 产品名称：
- APC anti-mouse Ly-6A/E (Sca-1) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 122511
- 产品应用：
- 122511
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- mouse pre-T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1,?immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.
The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7
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- Application References
(PubMed link indicates BioLegend citation) -
- Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
- Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
- van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
- van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
- Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
- Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
- Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
- Product Citations
-
- Almada AE, et al. 2021. Cell Reports. 34(4):108656. PubMed
- Xie T, et al. 2021. Sci Adv. 7: . PubMed
- Karra L, et al. 2020. Oncogene. 6920:39. PubMed
- Bultmann–Mellin I, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 313:L687. PubMed
- Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
- Britschgi A, et al. 2017. Nature. 541:541-545. PubMed
- Masschelein E, et al. 2020. Skelet Muscle. 10:21. PubMed
- Ogawa-Wong A, et al. 2022. Metabolites. 12:. PubMed
- Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
- Lopes C, et al. 2016. PLoS One. 11: 0162927. PubMed
- Simon M, et al. 2019. Cell Metab. 29:871. PubMed
- JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
- Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
- Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
- Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
- Sakai H, et al. 2022. iScience. 25:103928. PubMed
- Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
- Carmody C et al. 2019. Endocrinology. 160(5):1205-1222 . PubMed
- Siddique SM, et al. 2019. Sci Rep. 9:13977. PubMed
- Wang X, et al. 2018. Nat Commun. 9:551. PubMed
- An X, et al. 2020. Thyroid. . PubMed
- RRID
- AB_756196 (BioLegend Cat. No. 122511) AB_756197 (BioLegend Cat. No. 122512)
- Structure
- Ly-6 multigene family, 18 kD
- Distribution
- Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
- Function
- Regulates B and T cell responses
- Cell Type
- Hematopoietic stem and progenitors, T cells, B cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Antigen References
-
1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.
- Gene ID
- 110454 View all products for this Gene ID
- UniProt
- View information about Ly-6A/E on UniProt.org
