Biotin anti-human IL-34 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 176	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human IL-34

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5 - 2.0 ?g/mL is recommended. To obtain a linear standard curve, serial dilutions of human IL-34 recombinant protein ranging from 5000 to 78 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated E033E8 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified E0320E7 antibody (Cat. No. 361301) as the capture antibody and recombinant human IL-34 (Cat. No. 577909) as the standard.

Note: For testing human IL-34 in serum, plasma or cell culture supernatant, LEGEND MAX? Human IL-34 ELISA Kit with Pre-coated Plates (Cat. No. 439607 & 439608) are specially developed and recommended.

Product Citations

Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
Han N, et al. 2021. STAR Protocols. 2(2):100460. PubMed

RRID

AB_2563034 (BioLegend Cat. No. 361401) AB_2563035 (BioLegend Cat. No. 361402)

Antigen Details

Structure: Secreted glycoprotein, homodimer
Distribution: IL-34 mRNA is expressed in many different tissues including: spleen, heart, brain, lung, liver, kidney, thymus, testes, ovary, small intestine, prostate, and colon
Function: IL-34 increases monocyte viability, induces macrophage proliferation, and synergizes with other cytokines to generate macrophages and osteoclasts from cultured progenitors. IL-34 promotes the formation of the colony forming unit-macrophage (CFU-M) in human bone marrow culture. IL-34 supports RANKL-induced osteoclastogenesis by promoting the adhesion and proliferation of osteoclast progenitors. Human IL-34 promotes osteoclast differentiation from peripheral blood mononucleated cells. In addition, systematic administration of IL-34 to mouse increases the number of CD11b+ cells and reduces bone mass. IL-1? and TNF-?± induce the expression of interleukin-34 mRNA in osteoblasts. IL-34 is also induced in macrophages infected with equine infectious anemia virus (EIAV).
Interaction: Macrophages, monocytes, myeloid cells
Ligand/Receptor: CSF-1R
Biology Area: Cell Biology, Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Clanchy FI,?et al.?2006.?J. Leuko. Biol.?79:757.?
2. Lin H,?et al.?2008.?Science?320:807.?
3. Chihara T,?et al.?2010.?Cell. Death Differ.?17:1917.?
4. Wei S,?et al.?2010.?J. Leuko. Biol.?88:495.?
5. Eda H,?et al.?2011.?Rheumatol. Int.?31:1525.
Gene ID: 146433 View all products for this Gene ID
UniProt: View information about IL-34 on UniProt.org

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