PE/Fire™ 700 anti-mouse CD206 (MMR) Antibody
- 产品名称：
- PE/Fire™ 700 anti-mouse CD206 (MMR) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 141741
- 产品应用：
- 141741
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD206 (MMR)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Fire? 700 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
FC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire? 700 has a maximum excitation of 565 nm and a maximum emission of 695 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.
- Application References
(PubMed link indicates BioLegend citation) -
- Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
- Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
- Yang X, et al. 2015. PNAS. 112:2900. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- RRID
- AB_2922468 (BioLegend Cat. No. 141741)
- Structure
- Type I transmembrane protein, 175 kD, C-type lectin superfamily
- Distribution
-
Macrophages, dendritic cells, Langerhans cells, liver endothelial cells
- Function
- Pathogen recognition, endocytosis and phagocytosis, antigen presentation
- Ligand/Receptor
- Antigen containing mannose, fucose, or an N-acetyl glucosamine
- Cell Type
- Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- CD Molecules
- Antigen References
-
1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.
- Gene ID
- 17533 View all products for this Gene ID
- UniProt
- View information about CD206 on UniProt.org
