PE/Dazzle™ 594 anti-mouse CD206 (MMR) Antibo
- 产品名称：
- PE/Dazzle™ 594 anti-mouse CD206 (MMR) Antibo
- 产品类别：
- 抗体
- 产品编号：
- 141731
- 产品应用：
- 141731
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD206 (MMR)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
FC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.
- Application References
(PubMed link indicates BioLegend citation) -
- Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
- Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
- Yang X, et al. 2015. PNAS. 112:2900. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
- Zhang F, et al. 2019. Nat Commun. 10:3974. PubMed
- Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
- Paterson N, et al. 2022. Elife. 11:. PubMed
- Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
- Dangaj D, et al. 2019. Cancer Cell. 35:885. PubMed
- Cunha LD et al. 2018. Cell. 175(2):429-441 . PubMed
- Ahrends T, et al. 2022. STAR Protoc. 3:101157. PubMed
- Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
- Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
- RRID
- AB_2565931 (BioLegend Cat. No. 141731) AB_2565932 (BioLegend Cat. No. 141732)
- Structure
- Type I transmembrane protein, 175 kD, C-type lectin superfamily
- Distribution
-
Macrophages, dendritic cells, Langerhans cells, liver endothelial cells
- Function
- Pathogen recognition, endocytosis and phagocytosis, antigen presentation
- Ligand/Receptor
- Antigen containing mannose, fucose, or an N-acetyl glucosamine
- Cell Type
- Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- CD Molecules
- Antigen References
-
1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.
- Gene ID
- 17533 View all products for this Gene ID
- UniProt
- View information about CD206 on UniProt.org
