Brilliant Violet 650™ anti-mouse CD206 (MMR)
- 产品名称：
- Brilliant Violet 650™ anti-mouse CD206 (MMR)
- 产品类别：
- 抗体
- 产品编号：
- 141723
- 产品应用：
- 141723
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD206 (MMR)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650? under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
FC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.6 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 650? excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.
- Application References
(PubMed link indicates BioLegend citation) -
- Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
- Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
- Yang X, et al. 2015. PNAS. 112:2900. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Tu J, et al. 2022. Theranostics. 12:747. PubMed
- Zhou Y, et al. 2022. Nat Commun. 13:2906. PubMed
- Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
- Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed
- Ayaub E,et al. 2017. Sci Rep.. 10.1038/s41598-017-13511-z. PubMed
- Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
- Bachy S, et al. 2022. iScience. 25:103758. PubMed
- Eubler K, et al. 2021. Int J Mol Sci. 22:. PubMed
- Ayaub EA, et al. 2019. Immunol Cell Biol. 97:203. PubMed
- Petkevicius K, et al. 2019. Elife. 8:e47990. PubMed
- Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed
- Jablonski K, et al. 2015. PLoS One. 10: 0145342. PubMed
- Gangoso E, et al. 2021. Cell. 184:2454. PubMed
- Kumar P, et al. 2022. Nutrients. 14:. PubMed
- RRID
- AB_2562445 (BioLegend Cat. No. 141723)
- Structure
- Type I transmembrane protein, 175 kD, C-type lectin superfamily
- Distribution
-
Macrophages, dendritic cells, Langerhans cells, liver endothelial cells
- Function
- Pathogen recognition, endocytosis and phagocytosis, antigen presentation
- Ligand/Receptor
- Antigen containing mannose, fucose, or an N-acetyl glucosamine
- Cell Type
- Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction
- Molecular Family
- CD Molecules
- Antigen References
-
1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.
- Gene ID
- 17533 View all products for this Gene ID
- UniProt
- View information about CD206 on UniProt.org
