Purified anti-mouse IL-1α Antibody -上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 317	1

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli - expressed, recombinant mouse IL-1α
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ELISA Capture - Quality tested
WB, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5 - 2.0 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-1α recombinant protein ranging from 250 to 4 pg/ml are recommended for each ELISA plate. For Western Blotting applications, it is suggested to use 5 ?g antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: ELISA Capture: The purified BL1a-72 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated BL1a-89 antibody (Cat. Nos. 512503 & 512504) as the detection antibody and recombinant mouse IL-1a (Cat. No. 580309) as the standard.
RRID: AB_961376 (BioLegend Cat. No. 512402)

Structure: Cytokine; 17.4 kD (Mammalian)
Bioactivity: Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
Cell Sources: Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma,
Cell Targets: B cells, T cells, monocytes, fibroblasts
Receptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.
Regulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α₂-macroglobulin
Gene ID: 16175 View all products for this Gene ID
UniProt: View information about IL-1alpha on UniProt.org