Purified anti-human IL-8 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 106	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human IL-8, amino acids Ser28-Ser99 (Accession # NM_000584)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-8 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified BH0814 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated BH0840 (Cat. No. 514703/ 514704) antibody as the detecting antibody and recombinant human IL-8 (Cat. # 570909) as the standard.

Product Citations

Gruber CN, et al. 2020. Cell. 183:982. PubMed

RRID

AB_2028544 (BioLegend Cat. No. 514601) AB_2028546 (BioLegend Cat. No. 514602)

Antigen Details

Structure: CXC chemokine; 8.4 kD (Mammalian)
Ligand/Receptor: CXCR1 (CDw128); CXCR2
Bioactivity: Neutrophil/basophil chemoattractant; activates neutrophils, angiogenic factor
Cell Sources: Monocytes, lymphocytes, granulocytes, fibroblasts, endothelial cells, bronchial epithelial cells, keratinocytes, melanocytes, hepatocytes, mesangial cells, chondrocytes
Cell Targets: Neutrophils, basophils, lymphocytes
Receptors: CXCR1 (CDw128); CXCR2
Cell Type: Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Baggiolini M, et al. 1994. Adv. Immunol. 55:97.
3. Schr?der J, et al. 1992. Immunology Ser. 57:387.
4. Zwahlen R, et al. 1993. Intl. Rev. Expt. Pathol. 34:27.
Regulation: Upregulated by IL-1, IL-7, IL-17, IFN-γ, TNF-α, leukoregulin; downregulated by IL-4, TGF-β, glucocorticoids, vitamin D3
Gene ID: 3576 View all products for this Gene ID
UniProt: View information about IL-8 on UniProt.org

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