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Purified anti-NF-κB p65 Antibody
产品名称:
Purified anti-NF-κB p65 Antibody
产品类别:
抗体
产品编号:
611351
产品应用:
611351
[价格]
规格 价格 库存
25µg ¥ 120 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human NF-κB p65
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 0.50 ?g/mL.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 ?g/mL is suggested.?For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application

Application Notes

When testing by ICC, we recommend fixation with 4% PFA followed by permeabilization with Triton X-100 or ice-cold methanol.

Antigen Details

Structure
NF-κB p65 is a 551 amino acid protein with a predicted molecular weight of 60 kD.
Distribution

Ubiquitously expressed/ Cytoplasm and Nucleus

Function
Transcription, RAS pathway, host-virus interaction, oncogene
Interaction
NF-kappa-B, TLE5, TLE1, TP53BP2, GSK3B, MAP3K14, HDAC3, AKIP1, FOXP3
Biology Area
Cell Biology, Immuno-Oncology, Immunology, Innate Immunity, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References
  1. Giridharan S and Srinivasan M. 2018. J?Inflamm?Res. 11:407-419.
  2. Ren C, et al. 2021. Cell Death Differ. 29:381-392.
  3. Yu S, et al. 2020. FEBS Lett. 594:1368-1378.
Gene ID
NA
UniProt
View information about NF-kB p65 on UniProt.org

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