Purified anti-AKT Phospho (Ser473) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 118	1

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human AKT phosphorylated at Ser473
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 ?g/mL.?For immunocytochemistry, a concentration range of 2.5 - 5.0?μg/mL is recommended.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested by Western Blot using lysates prepared from HeLa cells untreated or treated with IGF-1, and NIH/3T3 cell untreated or treated with PDGF.?

This clone is suitable for ICC in cells treated with PFA and permeabilized with either methanol or Triton X-100.
RRID: AB_2924618 (BioLegend Cat. No. 606551) AB_2924618 (BioLegend Cat. No. 606552)

Antigen Details

Structure

AKT is a 480 amino acid protein with a predicted molecular weight of 56 kD

Distribution

Ubiquitously expressed, translocates to the membrane upon activation

Function

Catalytically inactive multimeric complex. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 by PDK2.

Interaction

Interacts through pleckstrin homology domain with second messengers. Interacts with AKTIP, CDKN1B.

Antigen References