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Purified anti-SARS-CoV-2 Nucleocapsid Antibody
产品名称:
Purified anti-SARS-CoV-2 Nucleocapsid Antibody
产品类别:
抗体
产品编号:
946101
产品应用:
946101
[价格]
规格 价格 库存
25µg ¥ 112 1

产品详情

Product Details

Verified Reactivity
SARS-CoV-2
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SARS-CoV-2 Nucleocapsid recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Direct ELISA,?ICFC?- Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 ?g/mL. For Direct ELISA application, a concentration of 3.7 ng/mL is recommended.?For?intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

SARS-CoV-2 is 90% homologous to SARS-CoV. ?Cross reactivity of anti-SARS-CoV-2 Nucleocapsid antibody (clone A20087H) with SARS-CoV has not been tested.

BioLegend offers two clones specific for SARS-CoV-2 nucleocapsid, clones A20087F and A20087H

  • Both clones have been verified for western blot, with A20087F showing a higher affinity for SARS-CoV-2 nucleocapsid for this application
  • Both clones have been verified for direct ELISA and displayed similar EC50 values for this application
  • A20087H has been verified for ICFC, while A20087F is not recommended for this application
RRID
AB_2892515 (BioLegend Cat. No. 946101) AB_2892515 (BioLegend Cat. No. 946102)

Antigen Details

Structure
SARS-CoV-2 is a 419 amino acid protein with an expected molecular weight of 46 kD
Distribution

Viral nucleoprotein, host cell Golgi apparatus

Function
RNA binding, transcription regulation
Antigen References
  1. Zeng W. et al. 2020. Biochem Biophys Res Commun 527(3):618-623.
  2. Yoshimoto FK. 2020. Protein J. 39(3):198-216.
  3. Cascarina SM. & Ross ED. 2020. The FACEB Journal. 34:9832-9842.
Gene ID
43740575 View all products for this Gene ID
UniProt
View information about Viral Protein on UniProt.org

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