Purified anti-AMPKα Phospho (Thr172) Antibod-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 141	1

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human AMPK alpha phosphorylated at Thr172
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125?- 1.0??g/mL.?For immunocytochemistry, a concentration?of 5.0?μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested for ICC using serum-starved untreated HEK293 cells (negative control) and HEK293 cells treated with 5.0 ?M oligomycin for 30 minutes (positive control). Staining was expected to localize to the nucleus and cytoplasm. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100.? Both PFA fixation followed by methanol permeabilization and PFA fixation followed by Triton X-100 permeabilization produced a strong signal with the expected localization. Methanol-only fixation only produced a faint signal.
RRID: AB_2904438 (BioLegend Cat. No. 600651) AB_2904438 (BioLegend Cat. No. 600652)