Purified anti-human cdc2 Phospho (Thr14) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µL	￥ 141	1

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human cdc2 phosphorylated at threonine 14
Formulation: Phosphate buffer, 0.5% BSA, 0.09% NaN₃
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.25 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:4000. For immunocytochemistry, a dilution of 1:50?is recommended. For?intracellular flow cytometric staining, the suggested dilution of this reagent is 1:100 per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is predicted to react with mouse cdc2 when phosphorylated at threonine 14 due to complete sequence homology between immunizing peptide and the cdc2 mouse orthologue.

This clone was tested for ICC using lambda protein phosphatase analysis and HeLa cells fixed with methanol or 4% PFA followed by permeabilization with either methanol or Triton X-100.?Only PFA fixation followed by methanol permeabilization produced phospho-specific staining of cdc2.
RRID: AB_2904458 (BioLegend Cat. No. 947401) AB_2904458 (BioLegend Cat. No. 947402)