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Purified anti-EGFR Antibody
产品名称:
Purified anti-EGFR Antibody
产品类别:
抗体
产品编号:
933901
产品应用:
933901
[价格]
规格 价格 库存
25µg ¥ 101 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide from human EGFR
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P, ICFC, IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 5 ?g/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 ?g per million cells in 100 ?L volume. For immunoprecipitation, the suggested use of this reagent is 2.5 ?g/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using 4% PFA-fixed A431 cells permabilized with methanol or Triton X-100. While both permeabilization methods were compatible with the antibody, methanol enabled superior staining.

This clone is predicted to recognize isoform I based off of complete sequence homology of the immunizing peptide and the corresponding region of the isoform.

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Product Citations
  1. Huang CC, et al. 2020. Biosens Bioelectron. 169:112362. PubMed
RRID
AB_2820215 (BioLegend Cat. No. 933901) AB_2820216 (BioLegend Cat. No. 933902)

Antigen Details

Structure
EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
Distribution

Plasma membrane/Ubiquitously expressed

Function
EGFR signaling, cell signaling
Cell Type
Endothelial cells, Epithelial cells
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family
CD Molecules, Growth Factors
Antigen References
  1. Hunter T and Cooper JA. 1981. Cell. 24:741.
  2. Gill GN and Lazar CS. 1981. Nature. 293:305.
  3. Reynolds FH, et al. 1981. Nature. 292:259
  4. Zhou M, et al. 2013. Cancer Res. 23:7056-67
Gene ID
1956 View all products for this Gene ID
UniProt
View information about EGFR on UniProt.org

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