Purified anti-RPS6 Phospho (Ser244) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 112	1

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human RPS6 phosphorylated at serine 244
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g/mL.?For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended.?For intracellular flow cytometry using our True-Phos? Perm Buffer, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: BioLegend offers two clones against this target, A18024A and A18024B:

For western blotting, A18024B displayed a higher affinity for RPS6 Phospho (Ser244) compared to A18024A. Both clones exhibit mouse reactivity.

For ICC, A18024B displayed a moderately higher affinity for RPS6 Phospho (Ser244) compared to A18024A. Both clones are mouse reactive for this application, and both clones are compatible with Triton X-100 and methanol permeabilization steps.

For ICFC, A18024A works in all three ICFC buffers (Cat# 425401, 421002, 424401). For ICFC, A18024A weakly stains mouse RPS6 Phospho (Ser244). A18024B is not recommended for ICFC due to high background staining.

Both clones are predicted to react with rat RPS6 when phosphorylated at serine 244 due to complete sequence homology between the immunizing sequence and the rat RPS6 ortholog.
RRID: AB_2832900 (BioLegend Cat. No. 935701) AB_2832901 (BioLegend Cat. No. 935702)